Supplemental Digital Content is available in the text. The protein p57 is encoded by CDKN1C. This gene is known to be paternally imprinted and maternally expressed in cytotrophoblasts and villous stromal cells. We present a method for evaluating p57 antibodies (Abs) in hydatidiform mole (HM) and demonstrate the results for 4 p57 Abs in various cell types. Five cases of complete HM, diploid with 2 paternal genome sets (CHM;PP), 5 cases of partial HM, triploid with 2 paternal and 1 maternal genome sets (PHM;PPM), and 5 cases of non-HM, with diploid biparental genomes (non-HM;PM) were stained with p57 Abs: 57P06, EP183, KP10, and KP39. Assessment of the fraction of nuclei stained, and the intensity of staining of the nuclei and cytoplasm was performed. For evaluation of the Abs, the observations in cytotrophoblasts, villous stromal cells, maternal decidual cells, and intermediate trophoblasts were scored. The fraction of stained nuclei in cytotrophoblasts and villous stromal cells and the staining of cytoplasm showed to be important parameters in the evaluation of the Abs. 57P06 was evaluated as optimal. KP10 showed moderate cytoplasmatic staining in maternal decidual cells and intermediate trophoblasts, and was evaluated as good. EP183 was evaluated as poor, primarily due to nuclear staining in ≥10% of the villous stromal cells in CHM;PP. KP39 was evaluated as poor, primarily due to strong cytoplasmatic staining in some cytotrophoblasts and villous stromal cells. A structured testing of p57 for diagnosing HM is recommended. No nuclear staining was observed in syncytiotrophoblasts of CHM;PP, indicating that in syncytiotrophoblasts also, CDKN1C is paternally imprinted.

中文翻译：

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葡萄胎中的 p57

文本中提供了补充数字内容。p57 蛋白由 CDKN1C 编码。已知该基因在细胞滋养层和绒毛基质细胞中具有父系印记和母系表达。我们提出了一种评估葡萄胎 (HM) 中 p57 抗体 (Abs) 的方法，并展示了各种细胞类型中 4 个 p57 Abs 的结果。完整 HM 5 例，具有 2 个父本基因组的二倍体（CHM；PP），5 例部分 HM，具有 2 个父本和 1 个母本基因组的三倍体（PHM；PPM），以及 5 例非 HM，具有二倍体双亲基因组（非 HM；PM）用 p57 Abs 染色：57P06、EP183、KP10 和 KP39。对染色的细胞核分数以及细胞核和细胞质的染色强度进行评估。为了评估 Abs，在细胞滋养层、绒毛基质细胞中的观察，对母体蜕膜细胞和中间滋养层进行评分。细胞滋养层和绒毛基质细胞中染色细胞核的比例和细胞质染色显示是评估 Abs 的重要参数。57P06 被评价为最佳。KP10 在母体蜕膜细胞和中间滋养细胞中显示出中度细胞质染色，并被评估为良好。EP183 被评估为差，主要是由于 CHM;PP 中≥10% 的绒毛基质细胞的核染色。KP39 被评估为差，主要是由于一些细胞滋养细胞和绒毛基质细胞中的强细胞质染色。建议对 p57 进行结构化测试以诊断 HM。在 CHM;PP 的合体滋养细胞中未观察到核染色，表明在合体滋养细胞中，CDKN1C 也有父系印记。和中间滋养层进行评分。细胞滋养层和绒毛基质细胞中染色细胞核的比例和细胞质染色显示是评估 Abs 的重要参数。57P06 被评价为最佳。KP10 在母体蜕膜细胞和中间滋养细胞中显示出中度细胞质染色，并被评估为良好。EP183 被评估为差，主要是由于 CHM;PP 中≥10% 的绒毛基质细胞的核染色。KP39 被评估为差，主要是由于一些细胞滋养细胞和绒毛基质细胞中的强细胞质染色。建议对 p57 进行结构化测试以诊断 HM。在 CHM;PP 的合体滋养细胞中未观察到核染色，表明在合体滋养细胞中，CDKN1C 也有父系印记。和中间滋养层进行评分。细胞滋养层和绒毛基质细胞中染色细胞核的比例和细胞质染色显示是评估 Abs 的重要参数。57P06 被评价为最佳。KP10 在母体蜕膜细胞和中间滋养细胞中显示出中度细胞质染色，并被评估为良好。EP183 被评估为差，主要是由于 CHM;PP 中≥10% 的绒毛基质细胞的核染色。KP39 被评估为差，主要是由于一些细胞滋养细胞和绒毛基质细胞中的强细胞质染色。建议对 p57 进行结构化测试以诊断 HM。在 CHM;PP 的合体滋养细胞中未观察到核染色，表明在合体滋养细胞中，CDKN1C 也有父系印记。