Acinetobacter baumannii is a nosocomial, opportunistic pathogen that causes several serious conditions including meningitis, septicemia, endocarditis, and pneumonia. It can be found in the oral biofilm, which may be a reservoir for pneumonia and chronic obstructive pulmonary disease. Subgingival colonization by A. baumannii is associated with chronic and aggressive periodontitis as well as refractory periodontal disease. Porphyromonas gingivalis, a keystone periodontal pathogen localized to subgingival plaque, is also implicated in several chronic conditions including aspiration pneumonia. Although both bacteria are found together in subgingival plaque and can cause multiple polymicrobial infections, nothing is known about the interactions between these two important human pathogens. In this study, we used RNA sequencing to understand the transcriptional response of both species as they adapt to heterotypic communities. Among the differentially regulated genes were those encoding a number of important virulence factors for both species including adhesion, biofilm formation, and protein secretion. Additionally, the presence of A. baumannii increased the abundance of P. gingivalis in model dual‐species communities. Collectively these results suggest that both P. gingivalis and A. baumannii adapt to each other and have synergistic potential for increased pathogenicity. In identifying the mechanisms that promote pathogenicity and refractory disease, novel approaches to mitigate polymicrobial synergistic interactions may be developed to treat or prevent associated diseases.

中文翻译：

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细菌群落中牙龈卟啉单胞菌和鲍曼不动杆菌的转录组分析。

鲍曼不动杆菌是一种医院机会病原体，可引起多种严重状况，包括脑膜炎，败血病，心内膜炎和肺炎。可以在口腔生物膜中找到它，它可能是肺炎和慢性阻塞性肺疾病的储存库。鲍曼不动杆菌的龈下定植与慢性和侵袭性牙周炎以及难治性牙周疾病有关。牙龈卟啉单胞菌牙周病原体，一种位于牙龈下斑块上的基石，也与包括吸入性肺炎在内的几种慢性疾病有关。尽管两种细菌都在龈下斑块中一起发现，并且可以引起多种微生物感染，但关于这两种重要的人类病原体之间的相互作用尚无定论。在这项研究中，我们使用RNA测序来了解这两个物种适应异型群落的转录反应。在差异调节的基因中，有一些编码两种物种的重要毒力因子，包括粘附，生物膜形成和蛋白质分泌。此外，鲍曼不动杆菌的存在增加了牙龈卟啉单胞菌的丰度。在双物种示范社区中。这些结果共同表明，牙龈卟啉单胞菌和鲍曼不动杆菌两者相互适应，并且具有增加致病性的协同潜力。在确定促进致病性和难治性疾病的机制时，可以开发减轻微生物协同相互作用的新方法来治疗或预防相关疾病。