Chromosome banding techniques were applied and standardized to obtain karyotype characteristics for the first time in Brazil of Nelore cattle - Bos taurus indicus Linnaeus, 1758 - (bovine subspecies most prominent in Brazilian livestock). Blood samples were collected from the animals of the School of Agrarian and Biological Sciences of the Pontifical Catholic University of Goiás, two males and two females of pure breed. These samples were submitted to the cell culture method to study metaphase chromosomes. Chromosome banding techniques (C, G and NOR) revealed the karyotype architecture of Nelore cattle common with that of other breeds of zebu cattle formerly karyotyped. The diploid chromosome number was invariably normal, 2n = 60. C-banding revealed C-positive heterochromatin in centromeric regions almost in all chromosomes. G-banding presented the expected band pattern in the respective chromosome pairs in correspondence with the established chromosomal patterns for the species. Ag-staining for nucleolus organizer regions (AgNOR) was identified on the telomeric end of the long arm in 7 autosomal chromosomes. In this study we found more regions in chromosomes with staining than presented in the literature for the Bos indicus group (BIN). These NOR regions were repeated on the same chromosomes for the 4 animals studied.

中文翻译：

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Nelore牛的带状核型（Bos taurus indicus Linnaeus，1758年）。

应用了染色体条带化技术并对其进行了标准化，以首次在巴西的内罗尔牛-Bos taurus indicus Linnaeus，1758年（在巴西牲畜中最突出的牛亚种）上获得核型特征。从戈亚斯天主教天主教大学农业和生物科学学院的动物中采集了血样，其中有两只雄性和两只雌性纯种。这些样品被送至细胞培养方法以研究中期染色体。染色体分带技术（C，G和NOR）揭示了内罗尔牛的核型结构与以前核型的其他种牛的核型结构相同。二倍体染色体数目始终是正常的，2n =60。C谱带显示几乎所有染色体中着丝粒区域的C阳性异染色质。G带显示了与该物种已建立的染色体模式相对应的各个染色体对中的预期带模式。在7条常染色体染色体的长臂端粒末端鉴定出了针对核仁组织区（AgNOR）的Ag染色。在这项研究中，我们发现染色体上带有染色的区域比文献中Bos indicus组（BIN）所示的区域更多。对于所研究的4只动物，这些NOR区域在相同的染色体上重复。在这项研究中，我们发现染色体上带有染色的区域比文献中Bos indicus组（BIN）所示的区域更多。对于所研究的4只动物，这些NOR区域在相同的染色体上重复。在这项研究中，我们发现染色体上带有染色的区域比文献中Bos indicus组（BIN）所示的区域更多。对于所研究的4只动物，这些NOR区域在相同的染色体上重复。