The motion of cells in a two-stream microfluidic device designed to extract cryoprotective agents from cell suspensions was tested under a range of conditions. Jurkat cells (lymphoblasts) in a 10% dimethylsulfoxide solution were driven in parallel with phosphate-buffered saline solution wash streams through single rectangular channel sections and multiple sections in series. The influence of cell-stream flow rate and cell volume fraction (CVF) on cell viability and recovery were examined. The channel depth was 500 lm, and average cell stream velocity within the channels was varied from 3.6 to8.5 mm/s corresponding with cell stream Reynolds numbers of 2.6-6.0. Cell viability measured at device outlets was high for all cases examined indicating no significant cell damage within the device. Downstream of a single stage, cell recoveries measured 90-100% for average cell stream velocities ≥6 mm/s and for CVFs up to 20%. Cell recovery downstream of multistage devices also measured 90-100% after a critical device population time. This time was found to be five times the average cell residence time within the device. The measured recovery values were significantly larger than those typically obtained using conventional cell washing methods.

中文翻译：

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两流微流控设备中的细胞运动和恢复。

在一系列条件下测试了设计用于从细胞悬液中提取冷冻保护剂的两流微流控设备中细胞的运动。将10％的二甲基亚砜溶液中的Jurkat细胞（成淋巴细胞）与磷酸盐缓冲盐溶液洗涤液流平行通过单个矩形通道部分和多个串联部分。检查了细胞流流速和细胞体积分数（CVF）对细胞活力和恢复的影响。通道深度为500 lm，通道内的平均细胞流速度在3.6至8.5 mm / s之间变化，对应于2.6-6.0的细胞流雷诺数。在所有检查的情况下，在设备出口处测得的细胞活力都很高，表明该设备内没有明显的细胞损伤。在单个阶段的下游，对于平均细胞流速度≥6mm / s和最高20％的CVF，细胞回收率测量为90-100％。在关键的设备填充时间之后，多级设备下游的细胞回收率也达到了90-100％。发现该时间是该装置内平均细胞停留时间的五倍。测得的恢复值明显大于使用常规细胞洗涤方法通常获得的恢复值。