Intestinal immunity and flora are reported to be associated with the onset of rheumatoid arthritis. However, differences in the intestinal immunity and flora dynamics between the initial peak and relapse of arthritis have not been investigated. Here we analyzed the lymphocyte populations in different lymphoid tissues, the IgA in feces, and the intestinal flora at the initial peak and the relapse phase of arthritis in a collagen-induced arthritis (CIA) mouse model. In this model compared with the control group, the percentage of RORγt+CD4+ T cells in the mesenteric lymph nodes (mLN) was increased at the initial peak but decreased at the relapse stage of arthritis, and the opposite changes were observed in the spleen. The percentage of Foxp3+CD4+ T cells was unchanged at the initial peak in both tissues but increased only in the mLN at the relapse stage. The IgA in feces increased with the progression of arthritis, and bacterial analysis revealed that some specific bacterial families were changed at the peak and relapse stages of arthritis. Finally, the immune dynamics under different arthritic conditions were examined by integrating these factors using principal component analysis (PCA). PCA showed that the immunological and intestinal flora profiles were different between the initial peak and the relapse of the arthritis. Our findings suggest that the intestinal immunity and the environment change drastically with the progress of arthritis.

中文翻译：

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胶原诱导的小鼠关节炎模型中肠道免疫和菌群分析：关节炎进展过程中的差异。

据报道，肠道免疫和菌群与类风湿关节炎的发作有关。但是，尚未研究关节炎的初始高峰和复发之间肠道免疫力和菌群动力学的差异。在这里，我们分析了胶原诱导的关节炎（CIA）小鼠模型中关节炎的初始峰和复发期的不同淋巴组织中的淋巴细胞数量，粪便中的IgA和肠道菌群。在该模型中，与对照组相比，肠系膜淋巴结（mLN）中的RORγt+ CD4 + T细胞百分比在初始高峰时增加，而在关节炎复发阶段则降低，在脾脏中观察到相反的变化。在两个组织中，Foxp3 + CD4 + T细胞的百分比在初始峰值均未改变，但在复发阶段仅在mLN中增加。粪便中的IgA随着关节炎的发展而增加，细菌分析表明，某些特定的细菌家族在关节炎的高峰期和复发期发生了变化。最后，通过使用主成分分析（PCA）整合这些因素来检查不同关节炎条件下的免疫动力学。PCA显示，关节炎的最初高峰和复发之间的免疫和肠道菌群特征不同。我们的发现表明，随着关节炎的进展，肠道免疫力和环境会发生巨大变化。细菌分析表明，某些特定的细菌家族在关节炎的高峰期和复发期发生了变化。最后，通过使用主成分分析（PCA）整合这些因素来检查不同关节炎条件下的免疫动力学。PCA显示，关节炎的最初高峰和复发之间的免疫和肠道菌群特征不同。我们的发现表明，随着关节炎的进展，肠道免疫力和环境会发生巨大变化。细菌分析表明，某些特定的细菌家族在关节炎的高峰期和复发期发生了变化。最后，通过使用主成分分析（PCA）整合这些因素来检查不同关节炎条件下的免疫动力学。PCA显示，关节炎的最初高峰和复发之间的免疫和肠道菌群特征不同。我们的发现表明，随着关节炎的进展，肠道免疫力和环境会发生巨大变化。PCA显示，关节炎的最初高峰和复发之间的免疫和肠道菌群特征不同。我们的发现表明，随着关节炎的进展，肠道免疫力和环境会发生巨大变化。PCA显示，关节炎的最初高峰和复发之间的免疫和肠道菌群特征不同。我们的发现表明，随着关节炎的进展，肠道免疫力和环境会发生巨大变化。