当前位置: X-MOL 学术J. Genet. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The evolution study on Oryza rufipogon. dw by whole-genome sequencing.
Journal of Genetics ( IF 1.4 ) Pub Date : 2019-09-24
Jilin Wang 1 , Song Yan , Shiyou Luo , Wei Deng , Xianhua Shen , Dazhou Chen , Hongping Chen
Affiliation  

The species of Oryza rufipogon. dw was first discovered at Dongxiang, Jiangxi in 1978. It is recognized as abundant in genetic resources with the characteristics of cold and insect resistance. A total of 100.15 Gb raw data was obtained from seven pair-end libraries by Illumina Hiseq4000 platform. Subsequently, a draft assembly genome of O. rufipogon. dw was generated with a final size of 422.7 Mb with a contig N50 of 15 kb and a scaffold N50 of 296.2 bb. The assembly genome size was higher than the estimated genome size (413 Mb) based on k-mer analysis. The identified repeat sequences accounted for 40.09% of the entire genome, and 32,521 protein-coding genes with an average of 4.59 exons per gene was annotated in five databases. Phylogenetic analysis using 1460 single-copy gene, O. rufipogon. dw was close with O. rufipogon by Bayes method. The wild rice species of O. rufipogon. dw divergence was estimated at ∼0.3 million years ago (Mya) from O. rufipogon, and ∼0.6 Mya from the O. sativa. The draft genome of O. rufipogon. dw provided an essential resource for its origin and evolution study.

中文翻译:

稻米rufipogon的进化研究。dw通过全基因组测序。

Oryza rufipogon的种类。dw于1978年首次发现于江西东乡。它被认为具有丰富的遗传资源,具有抗寒和抗虫的特性。Illumina Hiseq4000平台从七个配对末端库中获得了总计100.15 Gb的原始数据。随后,建立了红景天的装配基因组草图。dw的最终大小为422.7 Mb,重叠群N50为15 kb,支架N50为296.2 bb。装配的基因组大小高于基于k-mer分析的估计基因组大小(413 Mb)。鉴定出的重复序列占整个基因组的40.09%,并在五个数据库中标注了32,521个蛋白质编码基因,每个基因平均4.59个外显子。系统发育分析,使用1460单拷贝基因O. rufipogon。通过贝叶斯方法,dw与红景天很接近。rufipogon的野生稻种。dw的发散度据估计是由红景天(O. rufipogon)形成的,距今约30万年前(Mya),而来自紫花苜蓿的发散度约为0.6。红景天的基因组草图。dw为它的起源和进化研究提供了必要的资源。
更新日期:2019-11-01
down
wechat
bug