Cytoplasmic male sterility (CMS) is an important trait for large-scale hybrid seed production which avoids manual emasculation and undesired horizontal spread of pollen. Rearrangements in mitochondrial genome in terms of deletions and insertions are frequent causes leading to CMS. Mitochondrial ATP synthase is a multisubunit molecular machine which is involved in synthesis of ATP. In this study, three mutations in ATPase subunit 6 were identified and their cosegregation with male sterility was established using tobacco male sterile hybrids and Nicotiana suvaolensis. A breeder friendly Kompetitive allele specific polymerase chain reaction (KASP) SNP marker was developed for high throughput and quick genotyping. Introgression of this trait into selected germplasm lines (n = 9) was achieved based on foreground for CMS and background selection for recurrent parent using KASP marker and 50K custom tobacco SNP array, respectively. Analysis of genotyping data from SNP array revealed the presence of 88-99% of recurrent parent genome in BC3F1 plants. The selected BC3F1 plants exhibit CMS and are indistinguishable from the fertile recurrent parent (germplasm) in terms of plant morphology.

中文翻译：

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烟草细胞质雄性不育 KASP 标记的开发及其在性状渐渗中的应用。


细胞质雄性不育（CMS）是大规模杂交种子生产的一个重要性状，它可以避免人工去雄和花粉不希望的水平传播。线粒体基因组中缺失和插入的重排是导致 CMS 的常见原因。线粒体ATP合酶是参与ATP合成的多亚基分子机器。在这项研究中，鉴定了 ATP 酶亚基 6 的三个突变，并利用烟草雄性不育杂种和苏沃伦烟草建立了它们与雄性不育的共分离。开发了一种对育种者友好的竞争性等位基因特异性聚合酶链反应 (KASP) SNP 标记，用于高通量和快速基因分型。分别使用 KASP 标记和 50K 定制烟草 SNP 阵列，基于 CMS 的前景和轮回亲本的背景选择，实现了该性状向选定种质系 (n = 9) 的渗入。对 SNP 阵列的基因分型数据分析表明，BC3F1 植物中存在 88-99% 的轮回亲本基因组。所选的 BC3F1 植物表现出 CMS，并且在植物形态方面与可育轮回亲本（种质）没有区别。