BACKGROUND The scaffold for head and neck reconstruction needs mechanical strength to maintain specific forms. Hydroxyapatite (HA) enhances the mechanical strength of hydrogel and is routinely used for cartilage regeneration. However, there is a demand for hydroxyapatite that controls chondrogenic cell behavior. OBJECTIVE Our aim was to regulate HA morphology through a hydrothermal process using organic acid and enhance chondrocyte proliferation and differentiation using shaped-regulated HA. METHODS HA was synthesized from dodecanedioic acid (DD:HA) and oleic acid (OA:HA) by a hydrothermal method and then coated onto glass plates. Surface properties of the samples were compared by various techniques. Surface roughness and contact angles were calculated. Proliferation and differentiation of chondrogenic cells were measured by MTT assays and Alcian Blue staining, respectively, after various incubation periods. RESULTS The morphological structures of DD:HA and OA:HA were different; however, the crystallinity and chemical structures were similar. Surface roughness and hydrophilic behavior were higher on DD:HA. DD:HA enhanced chondrogenic cell proliferation over time. The differentiation of ATDC5 cells was also increased on the DD:HA surface compared with those in other groups. CONCLUSIONS DD:HA enhanced cell viability to a greater extent than OA:HA did, indicating its excellent potential as an inorganic material compatible with chondrocyte regeneration.

中文翻译：

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由十二烷二酸合成的板状羟基磷灰石可增强软骨细胞的增殖。

背景技术用于头部和颈部重建的支架需要机械强度以维持特定形式。羟基磷灰石（HA）增强了水凝胶的机械强度，通常用于软骨再生。然而，需要控制软骨细胞行为的羟基磷灰石。目的我们的目的是通过使用有机酸的水热过程来调节HA的形态，并使用形状调节的HA来增强软骨细胞的增殖和分化。方法采用水热法由十二烷二酸（DD：HA）和油酸（OA：HA）合成HA，然后涂在玻璃板上。通过各种技术比较样品的表面性质。计算表面粗糙度和接触角。在各种潜伏期后，分别通过MTT测定法和阿尔辛蓝染色法测定软骨细胞的增殖和分化。结果DD：HA和OA：HA的形态结构不同。但是，结晶度和化学结构相似。DD：HA的表面粗糙度和亲水性较高。DD：HA随着时间的推移增强了软骨细胞的增殖。与其他组相比，DD：HA表面上ATDC5细胞的分化也有所增加。结论DD：HA比OA：HA更大程度地增强了细胞活力，表明其作为与软骨细胞再生相容的无机材料具有出色的潜力。结晶度和化学结构相似。DD：HA的表面粗糙度和亲水性较高。DD：HA随着时间的推移增强了软骨细胞的增殖。与其他组相比，DD：HA表面上ATDC5细胞的分化也有所增加。结论DD：HA比OA：HA更大程度地增强了细胞活力，表明其作为与软骨细胞再生相容的无机材料具有出色的潜力。结晶度和化学结构相似。DD：HA的表面粗糙度和亲水性较高。DD：HA随着时间的推移增强了软骨细胞的增殖。与其他组相比，DD：HA表面上ATDC5细胞的分化也有所增加。结论DD：HA比OA：HA更大程度地增强了细胞活力，表明其作为与软骨细胞再生相容的无机材料具有出色的潜力。