Turkey has a rich freshwater biodiversity in terms of Cyprinid genus in respect to its geographical location. To elucidate the phylogeny of the Alburnoides genus, one of these genera, genetic data for the cytochrome b gene (1141 bp) was generated for 445 samples collected at 42 sampling sites across their geographical distribution. A total of 54 mitochondrial haplotypes identified were distrubuted among distinct twelve species that did not share haplotypes with each other. Pairwise sequence divergence among these species range from 1.37% (A. emineae and A. velioglui) and 10.99% (A. manyasensis and A. smyrnae). A new potential species in the River Dirgine that run into the Black Sea Basin was separated from the most closed known species with mean 6.3%. Network analysis and phylogenetic analysis indicated that all haplotypes were clustered into two major clades, which corresponded to twenty-three Alburnoides lineages, with moderate-high bootstrap supports and mutational steps, respectively. Application of a molecular clock to a Bayesian phylogeny indicates that Alburnoides diversified under the paleogeographic conditions such as tectonic uplift and faulting Miocene aged as well as climatic oscillation and sea-level fluctuations during late Miocene-middle Pleistocene. The genetic results of the present study indicated the inter-specific distance of cyt b gene sequences followed the ideal results for species identification and phylogeny of Turkish spirlins.

就其塞浦路斯地理位置而言，土耳其在塞浦路斯族中具有丰富的淡水生物多样性。为了阐明Alburnoides属的系统发育史，是其中一个属，在其地理分布中的42个采样点收集了445个样品的细胞色素b基因（1141 bp）遗传数据。总共鉴定出的54个线粒体单倍型分布在彼此不具有单倍型的不同的十二个物种中。这些物种之间的成对序列差异范围为1.37％（埃米农杆菌和A. velioglui）和10.99％（多米曲霉和S. smyrnae））。进入黑海盆地的迪尔吉内河中有一个新的潜在物种与已知最封闭的物种分离，平均比例为6.3％。网络分析和系统发育分析表明，所有单倍型都聚集成两个主要进化枝，分别对应于二十三个Alburnoides谱系，分别具有中等高的自举支持和突变步骤。将分子钟应用于贝叶斯系统发育研究表明，在古地理条件下，如高层抬升和中新世断层以及中新世-中更新世晚期的气候振荡和海平面波动等古地理条件下，Alburnoides多样化。本研究的遗传结果表明cyt b的种间距离 基因序列遵循了理想的结果，可用于土耳其螺螺菌种的鉴定和系统发育。