Beetles of the subfamily Scolytinae are the most damaging insects in the world. Among which the black twig borer, Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae: Scolytinae), is one of the serious pests in coffee plantations. Their cryptic life cycle inside the host plant makes these insects difficult to control. For its effective management accurate, timely and rapid identification of species is critical. By cloning and sequencing the 5' mitochondrial cytochrome oxidase c subunit 1 (COI) gene, the beetle’s molecular identification confirmed its identity as X. compactus. No pseudogenes and indels were found in analyzed nucleotide sequences; they match with high similarity in nucleotide NCBI Basic Local Alignment Search Tool search. The X. compactus COI genes sequences were deposited at NCBI GenBank with accession numbers of KY172634, KY172635 and the Barcode of Life (BOLD) with BIN ID: ACB4177. Furthermore, based on multiple sequence alignment of the X. compactus MtCOI gene, a phylogenetic tree with maximum probability was drawn. X. compactus species clustered together which agree with the species data collected from NCBI GenBank database from the different geographic regions. There were no morphological and molecular differences between space and time—collected coffee shot—hole borers, thus all the specimens described were X. compactus infesting both robusta and arabica coffee.

鞘翅目亚科的甲虫是世界上最具破坏性的昆虫。其中，黑树枝bore（Xichlosandrus compactus（Eichhoff）（鞘翅目：Curculionidae：Scolytinae））是咖啡种植园中的严重害虫之一。它们在寄主植物内部的神秘生命周期使这些昆虫难以控制。为了对其进行有效管理，准确，及时和快速地识别物种至关重要。通过克隆和测序5'线粒体细胞色素氧化酶c亚基1（COI）基因，甲虫的分子鉴定证实了它的致密性X.compactus。在分析的核苷酸序列中未发现假基因和插入缺失；它们在核苷酸NCBI基本本地比对搜索工具搜索中具有高度相似性。在十compactusCOI基因序列存放在NCBI GenBank中，登录号为KY172634，KY172635和生命条形码（BOLD），BIN ID：ACB4177。此外，基于紧凑型X. MtCOI基因的多序列比对，绘制了具有最大概率的系统树。X.紧凑虫物种聚集在一起，这与从NCBI GenBank数据库收集的来自不同地理区域的物种数据一致。在空间和时间（收集的咖啡粒）打孔器之间没有形态和分子上的差异，因此，所描述的所有标本均为致密罗布斯塔咖啡和阿拉比卡咖啡的致密线虫。