The liver is an important central organ, which controls carbohydrate metabolism through maintaining glucose homeostasis by a tightly regulated system of genes or enzymes. The microRNAs are small non-coding RNAs playing an important role in the regulation of genes associated with developmental biology, physiology, metabolism, etc. Thus, in this study, we have intended to detect liver-specific microRNAs in farmed carp, Labeo bata, upon being fed a diet with different levels of carbohydrates. Here, we have conducted the experiment for 45 days using fingerlings of farmed carp fed with 20% (control), 40%, and 60% gelatinized starch levels. The liver tissues were collected from each treatment and processed for RNA isolation, small RNA library preparation, and high-throughput sequencing using Illumina NexSeq500. Through sequencing, 15,779,417 reads in 20% CHO, 13,959,039 in 40% CHO, and 13,661,950 in 60% CHO reads were generated for control and treated fishes using three small RNA libraries. We have investigated 445 novel and 231 conserved microRNAs in 20%, 40%, and 60% carbohydrate (CHO), respectively, through computational analysis. The differential expression analysis of miRNAs was carried out between different treatments compared with control and this study depicted 117 known and 114 novel miRNA genes involved in carbohydrate metabolic pathways. Further, target prediction and gene ontology analysis revealed that miRNAs were involved in several pathways such as signaling pathway, G protein pathway, complement receptor–mediated pathway, dopamine receptor signaling pathway, epidermal growth factor pathway, and notch signaling pathway. The predicted miRNA sites in targeted genes were associated with cellular activities, developmental biology, DNA binding, Golgi apparatus, extracellular region, catalytic activity, MAPK cascade, etc. Overall, we have generated a vital resource of liver-specific miRNAs involved in metabolic gene regulation. These studies further will help develop miRNA inhibitors to study their role during carbohydrate metabolism in farmed carp.

中文翻译：

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使用高通量测序在养殖淀粉，鲤鱼的养殖鲤鱼中的肝脏特异性microRNA鉴定（Hamilton，1822）。

肝脏是重要的中央器官，它通过严格调控的基因或酶系统维持葡萄糖稳态来控制碳水化合物的代谢。microRNA是小的非编码RNA，在与发育生物学，生理学，代谢等相关的基因的调控中起着重要作用。因此，在本研究中，我们打算检测养殖鲤鱼，腊肠中肝脏特异性的microRNA。，在饮食中添加不同含量的碳水化合物。在这里，我们已经进行了45天的实验，使用了20％（对照），40％和60％糊化淀粉含量的养殖鲤鱼鱼种。从每种处理中收集肝脏组织，并进行处理以使用Illumina NexSeq500进行RNA分离，小RNA文库制备和高通量测序。通过测序，使用三个小RNA文库为对照和处理鱼生成了20％CHO中的15,779,417个读数，40％CHO中的13,959,039个读数和60％CHO中的13,661,950个读数。通过计算分析，我们分别研究了20％，40％和60％的碳水化合物（CHO）中的445个新颖的和231个保守的microRNA。与对照相比，在不同处理之间进行了miRNA的差异表达分析，该研究描述了涉及碳水化合物代谢途径的117个已知miRNA基因和114个新miRNA基因。此外，目标预测和基因本体分析表明，miRNA参与了多种途径，例如信号传导途径，G蛋白途径，补体受体介导途径，多巴胺受体信号传导途径，表皮生长因子途径和缺口信号途径。靶向基因中预测的miRNA位点与细胞活性，发育生物学，DNA结合，高尔基体，细胞外区域，催化活性，MAPK级联等相关。总的来说，我们已经获得了参与代谢基因的肝脏特异性miRNA的重要资源。规。