Accumulating evidence suggests that long noncoding RNAs (lncRNAs) are emerging as important regulators involved in diseases, including heart failure (HF). In this study, we used microarray profiles to examine the transcriptome of lncRNAs in left ventricle samples derived from HF patients. We designed a custom pipeline to reannotate lncRNAs from microarray data and identified a set of consistently dysregulated lncRNAs in HF across the three independent cohorts. In total, 84 lncRNAs were found to be consistently dysregulated in at least two cohorts. By using a rank aggregation method, we integrated correlated protein-coding genes of the consistently dysregulated lncRNAs derived from HF samples and characterized their biological functions based on the correlated genes. The transcriptional regulation relationships of these lncRNAs ranged from 104 to 261, suggesting their important regulatory functions. Among the conserved lncRNAs, AC018647.1 and AC009113.1 showed significant dysregulation across all three cohorts. Our results showed that the two lncRNAs were involved in development-associated and cardiac cycle-associated functions.

中文翻译：

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综合转录组分析揭示了长链非编码 RNA 的持续失调及其在心力衰竭中的转录调控关系。

越来越多的证据表明，长链非编码 RNA (lncRNAs) 正在成为参与疾病的重要调节因子，包括心力衰竭 (HF)。在这项研究中，我们使用微阵列谱来检查来自 HF 患者的左心室样本中 lncRNA 的转录组。我们设计了一个自定义管道来重新注释来自微阵列数据的 lncRNA，并在三个独立的队列中确定了一组在 HF 中持续失调的 lncRNA。总共发现 84 个 lncRNA 在至少两个队列中持续失调。通过使用秩聚合方法，我们整合了源自 HF 样本的持续失调的 lncRNA 的相关蛋白质编码基因，并基于相关基因表征了它们的生物学功能。这些 lncRNA 的转录调控关系从 104 到 261 不等，表明它们具有重要的调控功能。在保守的 lncRNA 中，AC018647.1和AC009113.1在所有三个队列中都显示出显着的失调。我们的结果表明，这两种 lncRNA 参与了发育相关和心动周期相关功能。