Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in the activity of excitatory and inhibitory systems, restores these functions and suppresses the progression of disability in experimental autoimmune encephalitis (EAE). The structural type of TMS, the arrangement as theta burst stimulation (TBS) has been applied as intermittent TBS (iTBS) and continuous TBS (cTBS) protocols to female adult DA rats. The animals were randomly divided into experimental groups: control group (C), group treated with complete Freund's adjuvant (CFA), experimental autoimmune encephalomyelitis (EAE) group, group treated with iTBS post EAE immunization (EAE + iTBS), group treated with cTBS post EAE immunization (EAE + cTBS), group of healthy animals treated with iTBS or cTBS. Therapeutic protocols of iTBS or cTBS in all EAE groups of animals were performed starting from 14 days post immunization (dpi), for 10 days with time point decapitation at 24 dpi. After decapitation, spinal cords were analysed for BDNF and Ki67 expression. The results revealed reduced BDNF expression in the rat's spinal cord of EAE animals in the stage of remission, which was associated with increased Ki67 and GFAP expressions. Decreased Iba 1 and BDNF expression, contrary to increased Iba 1 and Ki67 expression, suggests clustered microglia in the resolution phase of EAE. Enhanced GABA expression in spinal cord sections indicates higher GABA metabolic turnover, and also GAD activity in astrocytes, or prominent activity of GABAergic neurons. Both TBS protocols induced advance BDNF expression; amongst iTBS application provoked elevating of BDNF and stabilizing of GFAP and Ki67 expressions.

中文翻译：

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θ爆裂刺激影响实验性自身免疫性脑脊髓炎脊髓中BDNF的表达。

重复经颅磁刺激（rTMS）诱导参与兴奋性和抑制性系统活动的蛋白质表达变化，恢复这些功能并抑制实验性自身免疫性脑炎（EAE）的残疾发展。TMS的结构类型，θ爆裂刺激（TBS）安排已作为间歇性TBS（iTBS）和连续TBS（cTBS）方案应用于雌性成年DA大鼠。将动物随机分为实验组：对照组（C），完全弗氏佐剂（CFA）治疗组，实验性自身免疫性脑脊髓炎（EAE）组，EAE免疫后iTBS治疗组（EAE + iTBS），cTBS治疗组EAE免疫后（EAE + cTBS），用iTBS或cTBS治疗的健康动物组。iTBS或cTBS在所有EAE动物组中的治疗方案从免疫后（dpi）的14天开始进行10天，时间点断头为24 dpi。断头后，分析脊髓的BDNF和Ki67表达。结果表明，在缓解期，EAE动物的大鼠脊髓中BDNF表达降低，这与Ki67和GFAP表达增加有关。与升高的Iba 1和Ki67表达相反，降低的Iba 1和BDNF表达表明在EAE的分解期聚集的小胶质细胞。脊髓节段中GABA表达的增强表示GABA代谢新陈代谢较高，星形胶质细胞中GAD活性较高，或GABA能神经元的突出活性。两种TBS方案均可诱导BDNF的提前表达。