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Species identification of Indonesian agarwood using a DNA-barcoding method.
Journal of Natural Medicines ( IF 2.5 ) Pub Date : 2019-09-25 , DOI: 10.1007/s11418-019-01362-z
Seiji Tanaka 1 , Michiho Ito 1
Affiliation  

Agarwood is a type of resinous wood found in the trunks of Aquilaria and some other genera. It is widely used as an herbal medicine for sedation, detoxification, and treatment of stomachaches, as well as for incense sticks. However, the number of source plants is decreasing, and in 2005, they were added to Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). To identify source species of agarwood, we previously developed a DNA-barcoding method using resin deposition sites. In this study, to identify additional agarwood source species, the barcoding method was applied to source plants and commercial agarwood samples collected from Sumbawa, Lombok, Sulawesi, and Kalimantan in Indonesia, a major agarwood-producing country. In addition, the method was also applied to incense stick samples labeled as agarwood. As a result, several samples were identified as Gyrinops, which is not currently listed as an agarwood source plant in the Japanese standards for non-Pharmacopoeial crude drugs 2018 (Non-JPS 2018). From the viewpoint of securing future resources, these findings suggest that Gyrinops species should, therefore, be added to the list of agarwood source species.

中文翻译:

使用DNA条形码方法对印度尼西亚沉香树进行物种鉴定。

沉香是沉香树干中发现的一种树脂木和其他一些属。它被广泛用作镇静,排毒和治疗胃痛以及熏香的草药。然而,源植物的数量正在减少,2005年,它们被添加到《濒危野生动植物种国际贸易公约》(CITES)的附录II中。为了确定沉香木的来源种类,我们之前使用树脂沉积位点开发了一种DNA条形码方法。在这项研究中,为了识别其他沉香木来源物种,将条形码方法应用于从主要的沉香木生产国印度尼西亚的Sumbawa,Lombok,Sulawesi和Kalimantan收集的原始植物和商业沉香木样品。此外,该方法还适用于标记为沉香的香棒样品。结果,几个样品被鉴定为Gyrinops,目前尚未在2018年非药典原料药的日本标准中列为沉香木来源植物(Non-JPS 2018)。从获取未来资源的角度来看,这些发现表明,应该将紫罗兰属物种添加到沉香木源物种列表中。
更新日期:2019-09-25
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