In the past decade, different members of the genus Mamastrovirus have been associated with outbreaks of neurologic disease in humans, cattle, sheep, mink, and, most recently, porcine astrovirus 3 (PoAstV3) in swine. We performed a retrospective analysis of 50 cases of porcine neurologic disease of undetermined cause but with microscopic lesions compatible with a viral encephalomyelitis to better understand the role and pathogenesis of PoAstV3 infection. Nucleic acid was extracted from formalin-fixed paraffin-embedded (FFPE) tissue for reverse transcription quantitative polymerase chain reaction (RT-qPCR) testing for PoAstV3. In addition, 3 cases with confirmed PoAstV3-associated disease were assayed by RT-qPCR to investigate PoAstV3 tissue distribution. PoAstV3 was detected in central nervous system (CNS) tissue via RT-qPCR and in situ hybridization in 13 of 50 (26%) FFPE cases assayed. PoAstV3 was rarely detected in any tissues outside the CNS. Positive cases from the retrospective study included pigs in various production categories beginning in 2010, the earliest year samples were available. Based on these results, PoAstV3 appears to be a recurring putative cause of viral encephalomyelitis in swine that is rarely detected outside of the CNS at the time of clinical neurologic disease, unlike other common viral causes of neurologic disease in swine.

中文翻译：

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家猪中的脊髓灰质炎性脊髓炎与3型猪星状病毒有关。

在过去的十年中，马斯特罗病毒属的不同成员与人类，牛，绵羊，貂，以及最近在猪中的猪星状病毒3（PoAstV3）的神经系统疾病暴发有关。我们对50例病因未明但具有与病毒性脑脊髓炎相适应的微观病变的猪神经系统疾病进行了回顾性分析，以更好地了解PoAstV3感染的作用和发病机理。从福尔马林固定石蜡包埋的（FFPE）组织中提取核酸，用于PoAstV3的逆转录定量聚合酶链反应（RT-qPCR）测试。此外，通过RT-qPCR检测3例确诊为PoAstV3相关疾病的病例，以研究PoAstV3组织分布。通过RT-qPCR和50例FFPE病例中的13例（26％），在中枢神经系统（CNS）组织中检测到PoAstV3。在中枢神经系统外的任何组织中很少检测到PoAstV3。回顾性研究的阳性病例包括从2010年开始的各种生产类别的猪，最早的年份样本可用。根据这些结果，PoAstV3似乎是猪病毒性脑脊髓炎的复发推定原因，在临床神经系统疾病发生时，很少在CNS之外被发现，这与猪神经系统疾病的其他常见病毒原因不同。