Thecamoebida Smirnov and Cavalier-Smith, 2011 (Discosea, Amoebozoa) has been molecularly understudied. The group until recently consisted of three genera containing species that live in terrestrial or aquatic environments. Here, we describe a fourth genus, Stratorugosa tubuloviscum gen. nov. sp. nov., which was isolated from a freshwater Amoeba proteus Ward’s Science culture. Although this species most closely morphologically resembles a large, rugose Thecamoeba, S. tubuloviscum gen. nov. sp. nov. can be differentiated from Thecamoeba spp. by the following: 1) the presence of definitive finger-like (lobate-like) subpseudopodia extending at both the anterior and lateral parts of the cell during locomotion; 2) a peculiar locomotive mechanism with two sections, frontal and back, of the cells moving in a pulling and piggyback movement, respectively; 3) the presence of fibrillar cytoplasmic microtubules (MTs) organized by a prominent, perinuclear microtubule-organizing center (MTOC). A phylogenomic analysis of 511 genes assembled from transcriptomic data showed that this new genus was highly supported as sister to Stenamoeba. Despite the variance in gross morphology, Stenamoeba and S. tubuloviscum gen nov. sp. nov. both have MTOCs unlike two Thecamoeba spp., which display dot-like cytoplasmic MTs and lack an MTOC.

Thecamoebida Smirnov和Cavalier-Smith，2011年（迪斯科西，变形虫）在分子上研究不足。直到最近，该组由生活在陆地或水生环境中的三个属组成。在这里，我们描述了第四属，Stratorugosa tubuloviscum属。十一月 sp。nov。，它是从淡水变形虫变形菌Ward的科学文化中分离出来的。尽管该物种在形态上最接近于大型皱纹的Thecamoeba，S.tubuloviscum gen。十一月 sp。十一月 可以与Thecamoeba区分开spp。通过以下方法：1）在运动过程中，在细胞的前部和侧部均存在确定的手指状（叶状）假伪足；2）一种特殊的机车机构，其前部和后部两个部分分别以拉动和背负运动运动。3）由突出的核周微管组织中心（MTOC）组织的纤维状细胞质微管（MTs）的存在。从转录数据组装的511个基因的系统生物学分析表明，该新属作为茎线虫的姐妹得到了高度支持。尽管总体形态有所差异，但Stenamoeba和S.tubuloviscum gen nov。sp。十一月 都拥有不同于两个Thecamoeba的MTOC spp。，显示出点状胞质MT，但缺少MTOC。