This study was aimed at identifying differentially expressed genes (DEGs) with copy number changes in gastric cancer (GC) pathogenesis. Microarray data GSE33429, including array-based comparative genomic hybridization and gene expression profiles, were obtained. DEGs were screened between GC and adjacent noncancerous tissues. Genes located at Minimum Common Regions (MCRs) were identified, and overlapped genes between DEGs and genes with amplification or deletion were identified. Gene Ontology function and pathway enrichment analysis of DEGs were performed. A protein–protein interaction network for DEGs was built, and significant modules were mined from the network. Functional annotation of genes in modules was also performed. A total of 677 up- and 583 downregulated DEGs were identified, including 37 overexpressed genes located at gained MCRs and 28 downregulated genes located at deleted MCRs. In significant modules, upregulated genes with amplification, including DSN1 (MIS12 kinetochore complex component), MAPRE1 (microtubule-associated protein, RP/EB family, member 1), TPX2 (microtubule-associated), UBE2C (ubiquitin-conjugating enzyme E2C), and MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2), were associated with cell cycle, but downregulated genes with deletion, including UGT2B15 (UDP glucuronosyltransferase 2 family, polypeptide B15), UGT2B17 (UDP glucuronosyltransferase 2 family, polypeptide B17), ADH1B (alcohol dehydrogenase 1B), and ADH1A (alcohol dehydrogenase 1A), were related to metabolism. The identified genes DSN1, MAPRE1, TPX2, UBE2C, and MYBL2 located at gained MCRs and UGT2B15, UGT2B17, ADH1B, and ADH1A located at deleted MCRs may play an important role in GC progression through regulating cell cycle and metabolism.

中文翻译：

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DNA 拷贝数变化和基因表达的综合分析确定了胃癌的关键基因。

本研究旨在鉴定在胃癌 (GC) 发病机制中具有拷贝数变化的差异表达基因 (DEG)。获得了微阵列数据 GSE33429，包括基于阵列的比较基因组杂交和基因表达谱。在 GC 和邻近的非癌组织之间筛选 DEG。鉴定位于最小公共区域（MCRs）的基因，鉴定DEGs与扩增或缺失基因之间的重叠基因。进行了DEGs的基因本体功能和通路富集分析。建立了一个用于 DEG 的蛋白质-蛋白质相互作用网络，并从网络中挖掘出重要的模块。还进行了模块中基因的功能注释。共鉴定出 677 个上调和 583 个下调的 DEG，包括位于获得的 MCR 处的 37 个过表达基因和位于删除的 MCR 处的 28 个下调基因。在重要模块中，通过扩增上调基因，包括DSN1（MIS12 动粒复合物成分）、MAPRE1（微管相关蛋白，RP/EB 家族，成员 1）、TPX2（微管相关）、UBE2C（泛素结合酶 E2C）和MYBL2（v-myb 禽成髓细胞瘤病毒癌基因）同源物样 2)，与细胞周期相关，但下调基因缺失，包括UGT2B15（UDP 葡萄糖醛酸转移酶 2 家族，多肽 B15）、UGT2B17（UDP 葡萄糖醛酸转移酶 2 家族，多肽 B17）、ADH1B（酒精脱氢酶 1B）和ADH1A（酒精脱氢酶 1A），与代谢有关。鉴定的基因DSN1，MAPRE1、TPX2、UBE2C和MYBL2位于获得的 MCRs 和UGT2B15、UGT2B17、ADH1B和ADH1A位于缺失的 MCRs 可能通过调节细胞周期和代谢在 GC 进展中发挥重要作用。