SummaryReference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in mouse oocytes at the germinal vesicle (GV) stage. We have performed analysis of expression stability of the above-mentioned reference genes with the three most commonly used software tools: geNorm, BestKeeper and NormFinder. Taking into account the results obtained from all of these programmes Gapdh, Rpl4 and H2afz seem to be suitable candidate reference genes in GV oocytes of mouse.

中文翻译：

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小鼠生泡卵母细胞实时定量 PCR 分析的参考基因选择

摘要小鼠卵母细胞中的参考基因选择是对靶基因表达水平进行进一步充分分析所需的一项重要任务。在目前的工作中，我们分析了七个最常用的参考基因的表达稳定性（Actb、Eef1e1、Gapdh、H2afz、Ppia、Rpl4和英属哥伦比亚大学) 在生泡 (GV) 阶段的小鼠卵母细胞中。我们使用最常用的三种软件工具：geNorm、BestKeeper 和 NormFinder 对上述参考基因的表达稳定性进行了分析。考虑到从所有这些程序中获得的结果Gapdh，Rpl4和氢氟酸似乎是小鼠 GV 卵母细胞中合适的候选参考基因。