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Bordet-Gengou agar medium supplemented with albumin-containing biologics for cultivation of bordetellae.
Microbiology and Immunology ( IF 1.9 ) Pub Date : 2019-09-23 , DOI: 10.1111/1348-0421.12742
Yukihiro Hiramatsu 1 , Mayuko Osada-Oka 1, 2 , Yasuhiko Horiguchi 1
Affiliation  

Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections in mammals, including humans, and are generally cultivated on Bordet-Gengou (BG) agar plates in laboratories. The medium requires animal blood as a supplement for better bacterial growth. However, using blood is problematic, as its constant supply is occasionally difficult because of the limited shelf-life. This study proposes modified BG agar plates supplemented with bovine serum albumin and fetal bovine serum as a simple and convenient medium that confers sufficient growth of bordetellae.

中文翻译:

Bordet-Gengou琼脂培养基,添加了含白蛋白的生物制剂,用于培养紫菜科。

百日咳博德特氏菌,副百日咳博德特氏菌和支气管炎博德特氏菌在包括人在内的哺乳动物中引起呼吸道感染,通常在实验室的Bordet-Gengou(BG)琼脂平板上培养。该培养基需要动物血液作为补充,以促进更好的细菌生长。但是,使用血液是有问题的,因为由于有限的保存期限,有时很难稳定地供应血液。这项研究提出了改良的BG琼脂板,其中补充了牛血清白蛋白和胎牛血清,作为一种简单方便的培养基,可赋予波尔多菌足够的生长。
更新日期:2019-11-01
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