The efficient use of stem cells for transplantation is often limited by the relatively low number of stem cells collected. The ex vivo expansion of human stem cells for clinical use is a potentially valuable approach to increase stem cell number. Currently, most of the procedures used to expand stem cells are carried out using a 21% oxygen concentration, which is about 4- to 10-fold greater than the concentration characteristic of their natural niches. Hyperoxia might cause oxidative stress with a deleterious effect on the physiology of cultured stem cells. In this review, we investigate and critically examine the available information on the ability of natural compounds to counteract hyperoxia-induced damage in different types of stem cells ex vivo. In particular, we focused on proliferation and stemness maintenance in an attempt to draw up useful indications to define new culture media with a promoting activity on cell expansion in vitro.

中文翻译：

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天然化合物作为优化干细胞“体外”扩增的策略。

干细胞用于移植的有效利用通常受到收集的干细胞数量相对较少的限制。用于临床用途的人干细胞的离体扩增是增加干细胞数量的潜在有价值的方法。当前，用于扩增干细胞的大多数程序都是使用21％的氧气浓度进行的，该浓度比其天然小生境的浓度特征高约4至10倍。高氧血症可能导致氧化应激，对培养的干细胞的生理产生有害影响。在这篇综述中，我们调查并严格检查了有关天然化合物抵抗高氧诱导的不同类型干细胞体外损伤的能力的可用信息。。特别地，我们专注于增殖和干性维持，以试图画出有用的指示来定义对体外细胞扩增具有促进活性的新培养基。