Background: Mucuna pruriens Bak. Syn. Mucuna prurita Hook. seed is the rich source of levodopa (L-dopa) and has been used in traditional medicines to treat diseases resembling Parkinson’s disease. Objective: In the present study, a new HPTLC method was developed and validated for estimation of L-dopa from M. pruriens (black- and white-colored seeds) collected from two different locations in India. Method: TLC aluminum plates precoated with silica gel were used as the stationary phase. The plates were developed to a distance of 60 mm at a temperature of 22 ± 4°C in a twin glass chamber saturated with ethyl acetate–methanol–formic acid–water (15+3+3+4.5, v/v/v/v), as the mobile phase. Results: The R_f value of L-dopa was found to be 0.45. L-dopa was quantified at 282 nm, the wavelength of maximum absorbance by a densitometric scanner. The TLC plate was derivatized by ninhydrin reagent and photodocumented. L-dopa showed a good linearity in the concentration range 400–1000 ng/spot. The linear regression analysis of calibration plot showed good linear relationship between peak area and peak height (r² = 0.997). Conclusions: The method was validated for precision, repeatability, and accuracy. Recovery was determined by spiking L-dopa with samples and was found to be 96.10%. Highlights: L-dopa was found to be present at concentration 3.02–4.72% in samples and its formulation.

中文翻译：

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在Unani药物Mucuna pruriens Bak中左旋多巴的估计。高性能薄层色谱技术在市场上销售其制剂。

背景：Mucuna pruriens Bak。同步 Mucuna prurita钩。种子是左旋多巴（左旋多巴）的丰富来源，已被用于传统药物中，以治疗类似于帕金森氏病的疾病。目的：在本研究中，开发并验证了一种新的HPTLC方法，该方法可用于估计从印度两个不同地点收集的pr.ens（黑色和白色种子）中的L-多巴。方法：将预涂硅胶的TLC铝板用作固定相。在装有乙酸乙酯-甲醇-甲酸-水（15 + 3 + 3 + 4.5，v / v / v /）的双玻璃室中，将板在22±4°C的温度下显影至60 mm的距离v），作为流动相。结果：发现L-多巴的R _f值为0.45。L-多巴在光密度扫描仪在最大吸收波长282 nm处定量。用茚三酮试剂将TLC板衍生化并进行光记录。L-多巴在400-1000 ng / spot浓度范围内表现出良好的线性。校正图的线性回归分析显示峰面积和峰高之间具有良好的线性关系（r ² = 0.997）。结论：该方法经验证具有准确性，可重复性和准确性。通过向样品中掺入左旋多巴确定回收率，发现回收率为96.10％。要点：在样品及其制剂中，左旋多巴的浓度为3.02–4.72％。