The urinary excretion of hydroxyproline (Hyp), abundant in collagen protein, may serve as a biomarker of habitual collagen intake, assisting with investigations of current interest in the role of dietary collagen intake in supporting the synthesis of collagenous body tissues. This study investigated the time course of urinary Hyp excretion in "free-living," healthy, active males following the ingestion of a standardized bolus (20 g) of collagenous (gelatin and a hydrolyzed collagen powder) and dairy (calcium caseinate and hydrolyzed casein) proteins. The excretion of Hyp was assessed over a 24-hr period, separated into three collection periods: 0-6, 6-12, and 12-24 hr. Hyp was elevated for 0-6 hr after the consumption of collagen-containing supplements (gelatin 31.3 ± 8.8 mmol/mol and hydrolyzed collagen 33.7 ± 22.0 mmol/mol vs. baseline: gelatin 2.4 ± 1.7 mmol/mol and hydrolyzed collagen 2.8 ± 1.5 mmol/mol; p < .05), but not for the dairy protein supplements (calcium caseinate 3.4 ± 1.7 mmol/mol and hydrolyzed casein 4.0 ± 3.7 mmol/mol; p > .05). Therefore, urinary Hyp reflects an acute intake of collagenous protein, but is not suitable as a biomarker for quantifying habitual collagen intake, provided through regular dietary practices in "free-living," healthy, active males.

中文翻译：

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尿羟脯氨酸仅适合作为“自由生活”的健康，活跃男性中胶原蛋白张贴后长达6小时的急性摄入的生物标志物。

胶原蛋白含量丰富的羟脯氨酸（Hyp）的尿排泄物可以作为习惯性胶原摄入的生物标志物，有助于研究目前对膳食胶原摄入在支持胶原身体组织合成中的作用的研究。这项研究调查了摄入胶原蛋白（明胶和水解胶原蛋白粉）和乳制品（酪蛋白酸钙和酪蛋白水解钙）的标准大剂量（20克）后，“自由活动”健康，活跃的男性尿液Hyp排泄的时间过程。 ）蛋白质。在24小时内评估Hyp的排泄，分为三个收集时间段：0-6、6-12和12-24小时。食用含胶原蛋白的补充剂（明胶31.3±8.8 mmol / mol和水解胶原33.7±22.0 mmol / mol相对于基线）后，Hyp升高0-6小时：明胶2.4±1.7 mmol / mol和水解胶原蛋白2.8±1.5 mmol / mol; p <.05），但不适用于乳蛋白补充剂（酪蛋白酸钙3.4±1.7 mmol / mol和水解酪蛋白4.0±3.7 mmol / mol； p> .05）。因此，尿液Hyp反映了胶原蛋白的急性摄入，但不适合作为通过定量饮食习惯在“自由生活”的健康，活跃男性中提供的习惯性胶原摄入量的生物标记。