Information on the role of resistin on steroidogenesis is limited to animal studies. The aim of this study was to investigate the effect of various doses of resistin on estradiol and progesterone secretion from human luteinized granulosa cells in culture. Granulosa cells were obtained from follicular fluid aspirated from 50 women undergoing in vitro fertilization (IVF) treatment. The cells were cultured for 48 h after a 24 h pre-incubation period. The effect of resistin at dosages 1, 10 and 100 ng/ml alone or in combinations with FSH (10 and 100 ng/ml) on steroidogenesis was investigated. Estradiol and progesterone were measured by radioimmunoassays in culture supernatants at 24 h and 48 h. FSH treatment increased both estradiol and progesterone secretion. Resistin suppressed basal estradiol (at 1 ng/ml) and progesterone secretion (at all concentrations tested). When resistin (all concentrations) was combined with FSH (100 ng/ml), it eliminated the stimulatory effect of FSH on the secretion of estradiol and progesterone. This study indicates an inhibitory effect of resistin on the secretion of estradiol and progesterone by human luteinized granulosa cells in vitro. It is likely that this adipokine locally affects ovarian function in women.

Abbreviations: 3β-HSD: 3β-hydroxysteroid dehydrogenase; CAP1: cyclase-associated protein 1; DCN: decorin; FIZZ: Found in Inflammatory Zones; hCG: human chorionic gonadotropin; IGF1: insulin-like growth factor type 1; IVF: in vitro fertilization; PCOS: polycystic ovary syndrome; RIA: radioimmunoassay; ROR1: receptor tyrosine kinase-like orphan receptor-1; TLR4: Toll–like receptor 4

关于抵抗素在类固醇生成中作用的信息仅限于动物研究。这项研究的目的是研究不同剂量的抵抗素对培养物中人黄素化颗粒细胞中雌二醇和孕酮分泌的影响。颗粒细胞是从50名接受体外试验的妇女吸出的卵泡液中获得的受精（IVF）处理。预培养24小时后，将细胞培养48小时。研究了单独或与FSH（10和100 ng / ml）联合使用剂量分别为10、100和100 ng / ml的抵抗素对类固醇生成的影响。在24 h和48 h的培养上清液中通过放射免疫法测定雌二醇和孕酮。FSH治疗增加了雌二醇和孕酮的分泌。抵抗素抑制基础雌二醇（1 ng / ml）和孕酮分泌（在所有测试浓度下）。当抵抗素（所有浓度）与FSH（100 ng / ml）结合使用时，它消除了FSH对雌二醇和孕酮分泌的刺激作用。这项研究表明抵抗素对人黄体化颗粒细胞的体外雌二醇和孕酮分泌的抑制作用。该脂肪因子可能会局部影响女性的卵巢功能。

缩写： 3β-HSD：3β-羟基类固醇脱氢酶；CAP1：环化酶相关蛋白1；DCN：decorin; FIZZ：见于炎症区域；hCG：人绒毛膜促性腺激素；IGF1：1型胰岛素样生长因子；试管婴儿：体外受精；PCOS：多囊卵巢综合征；RIA：放射免疫分析；ROR1：受体酪氨酸激酶样孤儿受体1；TLR4：收费型受体4