Recent revolutionary advancements in sequencing technologies have made it possible to obtain mass quantities of genome-scale sequence data in a cost-effective manner and have drastically altered molecular biological studies. To utilize these sequence data, genome-wide association studies (GWASs) have become increasingly important. Hence, there is an urgent need to develop a visualization tool that enables efficient data retrieval, integration of GWAS results with diverse information and rapid public release of such large-scale genotypic and phenotypic data. We developed a web-based genome browser TASUKE+ (https://tasuke.dna.affrc.go.jp/), which is equipped with the following functions: (i) interactive GWAS results visualization with genome resequencing data and annotation information, (ii) PCR primer design, (iii) phylogenetic tree reconstruction and (iv) data sharing via the web. GWAS results can be displayed in parallel with polymorphism data, read depths and annotation information in an interactive and scalable manner. Users can design PCR primers for polymorphic sites of interest. In addition, a molecular phylogenetic tree of any region can be reconstructed so that the overall relationship among the examined genomes can be understood intuitively at a glance. All functions are implemented through user-friendly web-based interfaces so that researchers can easily share data with collaborators in remote places without extensive bioinformatics knowledge.

中文翻译：

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TASUKE+：一个基于网络的平台，用于探索全基因组关联研究结果和大规模重测序数据。

最近测序技术的革命性进步使得以经济有效的方式获得大量基因组规模的序列数据成为可能，并极大地改变了分子生物学研究。为了利用这些序列数据，全基因组关联研究（GWAS）变得越来越重要。因此，迫切需要开发一种可视化工具，能够实现高效的数据检索、GWAS结果与多样化信息的整合以及大规模基因型和表型数据的快速公开发布。我们开发了一个基于网络的基因组浏览器TASUKE+（https://tasuke.dna.affrc.go.jp/），它配备了以下功能：（i）交互式GWAS结果可视化，包含基因组重测序数据和注释信息，（ ii) PCR 引物设计，(iii) 系统发育树重建和 (iv) 通过网络共享数据。GWAS 结果可以以交互式和可扩展的方式与多态性数据、读数深度和注释信息并行显示。用户可以为感兴趣的多态性位点设计 PCR 引物。此外，还可以重建任意区域的分子系统发育树，使所检查的基因组之间的整体关系一目了然。所有功能都是通过用户友好的基于网络的界面实现的，因此研究人员可以轻松地与远程合作者共享数据，而无需丰富的生物信息学知识。