A glioma is a malignant tumor that severely threatens human health. However, it is difficult for most therapeutic agents to penetrate through the blood-brain barrier (BBB) and exhibit their antineoplastic activity in the brain. In this article, a biomimetic in vitro BBB model was created by a composite process, this model can provide a significant foundation for the research of drug transport, tumor treatment, tumor microenvironment and other fields. A series of tests and comparative experiments were performed to evaluate this model. The tests showed that the model enabled preliminary simulation of the structure and function of the BBB. Experimental results demonstrated: (1) the new technology enabled controlled release of growth factors and successfully induced endothelial progenitor cells into endothelial cells. Compared with the traditional gold standard, the Transwell model, the expression of four specific proteins that are related to the BBB characteristics was significantly increased (alkaline phosphatase(ALP) by 89.82%, γ-GT by 88.86%, zonula occludens-1 (ZO-1) by 57.40%, and Claudin-5 by 102.32%) in this model; (2) astrocytes had a promoting effect on the microvascular endothelial cells to form tight junction (ZO-1 increased by 249.35%, Claudin-5 increased by 184.99%), and there was a great difference between whether these two types of cells were contact cultured or not; (3) the gelatinous cell U118 had a destructive effect on the tight junction of BBB (ZO-1 decreased by 55.86%, Claudin-5 decreased by 37.84%).

中文翻译：

---

新型体外仿生血脑屏障模型的生物制造。

神经胶质瘤是严重威胁人类健康的恶性肿瘤。但是，大多数治疗剂很难穿过血脑屏障（BBB）并在大脑中表现出抗肿瘤活性。本文通过复合工艺建立了仿生体外BBB模型，该模型可为药物转运，肿瘤治疗，肿瘤微环境等领域的研究提供重要的基础。进行了一系列测试和比较实验以评估该模型。测试表明，该模型可以对BBB的结构和功能进行初步仿真。实验结果表明：（1）这项新技术能够控制生长因子的释放，并成功地诱导内皮祖细胞进入内皮细胞。与传统的金标准Transwell模型相比，与BBB特性相关的四种特定蛋白的表达显着增加（碱性磷酸酶（ALP）升高89.82％，γ-GT升高88.86％，小带闭合1（ZO在此模型中，-1）降低了57.40％，Claudin-5降低了102.32％）；（2）星形胶质细胞对微血管内皮细胞形成紧密连接有促进作用（ZO-1增加249.35％，Claudin-5增加184.99％），并且这两种类型的细胞是否接触有很大差异。是否培养 （3）胶质细胞U118对BBB的紧密连接有破坏作用（ZO-1降低55.86％，Claudin-5降低37.84％）。与BBB特征相关的四种特定蛋白的表达显着增加（碱性磷酸酶（ALP）增加了89.82％，γ-GT增加88.86％，小带闭合1（ZO-1）增加了57.40％和Claudin-5 102.32％）；（2）星形胶质细胞对微血管内皮细胞形成紧密连接有促进作用（ZO-1增加249.35％，Claudin-5增加184.99％），并且这两种类型的细胞是否接触有很大差异。是否培养 （3）胶质细胞U118对BBB的紧密连接有破坏作用（ZO-1降低55.86％，Claudin-5降低37.84％）。与BBB特征相关的四种特定蛋白的表达显着增加（碱性磷酸酶（ALP）增加了89.82％，γ-GT增加88.86％，小带闭合1（ZO-1）增加了57.40％和Claudin-5 102.32％）；（2）星形胶质细胞对微血管内皮细胞形成紧密连接有促进作用（ZO-1增加249.35％，Claudin-5增加184.99％），并且这两种类型的细胞是否接触有很大差异。是否培养 （3）胶质细胞U118对BBB的紧密连接有破坏作用（ZO-1降低55.86％，Claudin-5降低37.84％）。（2）星形胶质细胞对微血管内皮细胞形成紧密连接有促进作用（ZO-1增加249.35％，Claudin-5增加184.99％），并且这两种类型的细胞是否接触有很大差异。是否培养 （3）胶质细胞U118对BBB的紧密连接有破坏作用（ZO-1降低55.86％，Claudin-5降低37.84％）。（2）星形胶质细胞对微血管内皮细胞形成紧密连接有促进作用（ZO-1增加249.35％，Claudin-5增加184.99％），并且这两种类型的细胞是否接触有很大差异。是否培养 （3）胶质细胞U118对BBB的紧密连接有破坏作用（ZO-1降低55.86％，Claudin-5降低37.84％）。