The biochemical properties and biological activities of the venom from three individual Ophiophagus hannah (King cobra) specimens was compared. The toxicity against mice, the cytotoxicity against five cell lines, and the antioxidant activity were measured. The KV2 venom showed a higher cytotoxicity than the KV6 and the non-cytotoxic KV9 venoms. Comparative analysis of the O. hannah venom proteins was performed after 2-dimensional (2-D) denaturing gel electrophoresis and reverse phase high performance liquid chromatography (RP-HPLC). 2-D analysis by isoelectric focusing (IEF) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) resolution of the venoms revealed significant differences between all three venoms, with most spots being unique to that venom. Only 2 out of the 13–16 distinct spots were common to all three venoms, and four spots were common to KV6 and KV9. KV2 had the highest proportion of low molecular mass spots, and KV6 and KV9 appeared more related to each other than to KV9. From peptide mass mapping by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and MASCOT-based amino acid sequence database searching, the two venom proteins that were common to all three specimens are likely to be ophanin and acidic phospholipase A₂ (PLA₂), whilst the proteins unique to the cytotoxic KV2 venom, included three other PLA₂ proteins. The RP-HPLC pattern of KV2 was different from the other two venoms with a higher protein concentration eluting in the 31–41% (v/v) acetonitrile (ACN) fraction than for the other two venoms.

中文翻译：

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眼镜王蛇（Ophiophagus hannah）毒液的蛋白质组成和生物活性的变化。

比较了三个单独的Ophiophagus hannah（眼镜王蛇）标本中毒液的生化特性和生物学活性。测量对小鼠的毒性，对五种细胞系的细胞毒性和抗氧化活性。KV2毒液显示出比KV6和非细胞毒KV9毒液更高的细胞毒性。O. hannah的比较分析在二维（2-D）变性凝胶电泳和反相高效液相色谱（RP-HPLC）之后，进行蛇毒蛋白的纯化。通过等电聚焦（IEF）进行的2-D分析表明，十二烷磺酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分辨率显示出这三种毒液之间的显着差异，其中大多数斑点是该毒液所独有的。在13-16个不同的斑点中，只有两个是所有三个毒液共有的，而四个斑点是KV6和KV9共有的。KV2具有最高比例的低分子量斑点，并且KV6和KV9彼此之间的相关性高于与KV9的相关性。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和基于MASCOT的氨基酸序列数据库搜索进行肽质量定位，₂（PLA ₂），而具有细胞毒性KV2毒液的独特蛋白包括其他三种PLA ₂蛋白。KV2的RP-HPLC模式与其他两种毒液不同，在31–41％（v / v）乙腈（ACN）组分中洗脱的蛋白质浓度高于其他两种毒液。