The genus Pristimantis has the highest species diversity among all terrestrial vertebrates, with most species observed in the Andean region and the Guiana Shield. Even with the recent description of a new species, only P. latro, P. dundeei and P. zimmermanae occur in the south of the Amazon River. The lack of taxonomists specialized in the field leads to the propagation of dubious terminologies (e.g. Pristimantis sp1, Pristimantis sp2, P. aff. Fenestratus and P. gr. conspicillatus) or even misidentification of species, resulting in erroneous species distributions. In this study, we applied the Automatic Barcode Gap Discovery (ABGD) algorithm for the delimitation of candidate species and values of genetic distances using the mitochondrial marker Cytochrome Oxidase Subunit I (COI), proposed in the barcode methodology, where values greater than 10% are considered as indicative of different species. We found large genetic distances between P. latro and Pristimantis sp1 Unconfirmed Candidate Species – UCS1 (21%), and between P. altamazonicus and Pristimantis sp2 UCS2 (14%). The ABGD method recognized UCS1 and UCS2 as distinct species. Pristimantis sp. UCS1 and UCS2 in the east of the Brazilian Amazon are indicated as candidate species. We suggest greater sampling of Pristimantis sp. UCS1 and UCS2, integrating morphology and bioacoustics to solve the taxonomic status in the east of the Brazilian Amazon.

属Pristimantis拥有所有陆地脊椎动物中最高的物种多样性，与安第斯地区和圭亚那地盾观察种类最多。尽管最近一个新物种的描述中，仅P.拉特罗，P. dundeei和P. zimmermanae发生在亚马逊河的南部。缺乏分类学家的专业领域导致可疑的术语的传播（例如Pristimantis SP1，Pristimantis SP2，P. AFF。孔原黑丽鱼和P.克。Ç onspicillatus），甚至错误地识别物种，从而导致错误的物种分布。在这项研究中，我们使用条形码方法中提出的线粒体标记细胞色素氧化酶亚基I（COI），应用自动条形码间隙发现（ABGD）算法来划定候选物种和遗传距离值，该值大于10％被认为是不同物种的指示。我们发现在La。Latro和Pristimantis sp1未确认的候选物种UCS1之间存在较大的遗传距离（21％），在Altamazonicus和Pristimantis sp2 UCS2之间（14％）存在较大的遗传距离。ABGD方法将UCS1和UCS2识别为不同的物种。Pristimantissp。巴西亚马逊东部的UCS1和UCS2被指定为候选物种。我们建议对Pristimantis sp。进行更多采样。UCS1和UCS2，整合了形态学和生物声学特性，以解决巴西亚马逊东部的生物分类地位。