Circulating exosomes are promising biomarker source in various diseases. Exosomal constituents can stably exist in the circulating plasma and serum thus making them ideal biomarkers for a number of clinical applications. Exosomes can also mediate the occurrence of many types of diseases, including distal cancerous metastasis and tumour enlargement, through encapsulated proteins or RNAs, which regulate interactions among tissues. While performing these actions, exosomes show tissue specificity. However, the mechanism for such selection is not clear. For non-small cell lung cancer (NSCLC), molecular diagnostic markers and mechanisms of exosome-mediated tumour metastasis are not well understood. Therefore, in this study, we characterized LLC exosomal proteins and mRNAs by analysing their molecular profiles, laying a foundation for exploring diagnostic markers of lung cancer. Furthermore, the interactions between exosomal membrane proteins and their target proteins were analysed and revealed a possible tissue propensity of LLC cell-derived exosomes. These findings provide a theoretical basis for studying exosome-mediated tissue targeting and distal lung cancer metastasis.

中文翻译：

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LLC外泌体的蛋白质谱和mRNA表达的表征。

循环外泌体是各种疾病中有希望的生物标志物来源。外泌体成分可以稳定存在于循环血浆和血清中，因此使它们成为许多临床应用的理想生物标记。外来体还可以通过调节组织间相互作用的封装蛋白或RNA介导许多类型的疾病的发生，包括远端癌转移和肿瘤扩大。在执行这些动作时，外泌体显示出组织特异性。但是，这种选择的机制尚不清楚。对于非小细胞肺癌（NSCLC），外泌体介导的肿瘤转移的分子诊断标志物和机制尚不清楚。因此，在这项研究中，我们通过分析LLC外泌体蛋白和mRNA的分子特征来表征它们，为探索肺癌的诊断标志物奠定基础。此外，分析了外泌体膜蛋白与其靶蛋白之间的相互作用，并揭示了LLC细胞衍生的外泌体的可能组织倾向。这些发现为研究外泌体介导的组织靶向和远处肺癌转移提供了理论基础。