This study was aimed to identify novel miRNA biomarkers and explore the cooperative function of multi-RNAs in the progress of primary melanoma. The miRNA expression profile GSE62370 generated from 9 congenital nevi and 92 primary melanoma samples was downloaded from the Gene Expression Omnibus database. Differentially expressed miRNAs between primary melanoma and congenital nevi were compared and the target genes of them were selected. Pathway enrichment analysis and protein/protein interaction (PPI) network of miRNA target genes were performed. In addition, the differential expression of miRNAs to identify the tumor stage-dependent differences in miRNA expression was analyzed. Differentially expressed miRNAs, including 6 upregulated and 23 downregulated, were found in primary melanoma. Besides, the miRNA-associated gene regulatory network revealed 274 nodes, including miR-142-5p and miR-125b, and 307 miRNA-target pairs. miRNA-related Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, such as melanoma, was found. Target genes in the PPI module were mainly enriched in cancer-related pathways. Finally, the melanoma stage-related overexpressed miR-142-5p and the downregulated miR-550, miR-1826, miR-1201, miR-205, and miR-125b were identified. Some validated miRNAs, including miR-125a/b, let-7a/b, and miR-205, were found and illustrated the reliability of our study. Four novel miRNAs, including miR-142-5p, miR-550a, miR-1826, and miR-1201, were considered to have potential prognostic values for primary melanoma.

中文翻译：

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已鉴定出用于原发性黑素瘤的新型MicroRNA生物标记物miR-142-5p，miR-550a，miR-1826和miR-1201。

这项研究旨在鉴定新的miRNA生物标志物，并探讨多RNA在原发性黑素瘤进展中的协同功能。从9个先天性痣和92个原发性黑色素瘤样品中产生的miRNA表达谱GSE62370从Gene Expression Omnibus数据库下载。比较原发性黑色素瘤和先天性痣之间差异表达的miRNA，并选择它们的靶基因。进行了miRNA靶基因的途径富集分析和蛋白质/蛋白质相互作用（PPI）网络。另外，分析了miRNA的差异表达以鉴定miRNA表达中的肿瘤阶段依赖性差异。在原发性黑素瘤中发现了差异表达的miRNA，包括6个上调和23个下调。除了，miR-142-5p和miR-125b，以及307个miRNA-target对。发现了与miRNA有关的《京都基因与基因组百科全书》（KEGG）途径，例如黑素瘤。PPI模块中的靶基因主要富集于癌症相关途径。最后，鉴定出与黑素瘤阶段相关的过表达的miR-142-5p和下调的miR-550，miR-1826，miR-1201，miR-205和miR-125b。找到了一些经过验证的miRNA，包括miR-125a / b，let-7a / b和miR-205，并说明了我们研究的可靠性。四个新颖的​​miRNA，包括miR-142-5p，miR-550a，miR-1826和miR-1201被认为对原发性黑素瘤具有潜在的预后价值。