Lipids that are labeled with the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) group are widely used as fluorescent analogues of native lipids in biological and model membranes to monitor a variety of processes. NBD-labeled lipids have previously been used to monitor the organization and dynamics of molecular assemblies such as membranes, micelles and reverse micelles utilizing the wavelength-selective fluorescence approach. In this paper, we have characterized the organization and dynamics of various NBD-labeled lipids using red edge excitation shift (REES) and other fluorescence approaches which include analysis of membrane penetration depths of the NBD group using the parallax method. We show here that the environment and location experienced by the NBD group of the NBD-labeled lipids could depend on the ionization state of the lipid. This could have potentially important implications in future studies involving NBD-labeled lipids as tracers in a cellular context.

中文翻译：

---

N-（7-硝基苯-2-氧杂-1,3-二氮杂-4-基）标记的脂质的组织和动力学：荧光方法。

带有NBD（7-硝基苯-2-氧杂-1,3-二氮杂-4-基）基团的脂质被广泛用作生物和模型膜中天然脂质的荧光类似物，以监测各种过程。NBD标记的脂质以前已使用波长选择性荧光方法用于监测分子组件（例如膜，胶束和反胶束）的组织和动力学。在本文中，我们使用红边激发位移（REES）和其他荧光方法（包括使用视差法分析NBD组的膜穿透深度）表征了各种NBD标记脂质的组织和动力学。我们在这里显示NBD标记的脂质的NBD组所经历的环境和位置可能取决于脂质的电离状态。