We here present novel insights into the dynamic changes of a nanosized lipid film during enzymatic degradation. When adding an aqueous solution containing a triacylglycerol lipase to an approximately 100nm thin triolein film, which is supported on a hard surface, the film thickness, elasticity, viscosity, and chemical composition were obtained continuously. Both a mechanical technique (quartz crystal microbalance with dissipation monitoring) and a spectroscopic technique (attenuated total reflection Fourier transform infrared spectroscopy) were utilised for this study. Detailed data revealed the effects of pH, Ca(2+), and catalytic rate on lipolysis, including product release from the film. It was found that under basic conditions and without Ca(2+), the lipolytic activity commence instantaneously upon addition of enzyme, whereas product release from the substrate film awaits conditions that favours release. A model for removal of degradation products from the film is introduced, including a novel interpretation of the lag phase phenomenon.

中文翻译：

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三酰基甘油薄膜脂解的滞后相和水解机理。

我们在这里提出了新的见解，对酶降解过程中纳米脂质膜的动态变化。当将包含三酰基甘油脂酶的水溶液添加到支撑在硬表面上的约100nm的三油精薄膜时，连续获得膜厚度，弹性，粘度和化学组成。这项研究既使用了机械技术（具有耗散监测功能的石英晶体微天平）又使用了光谱技术（衰减全反射傅里叶变换红外光谱法）。详细数据揭示了pH，Ca（2+）和催化速率对脂解的影响，包括产品从薄膜中的释放。发现在碱性条件下且没有Ca（2+）时，添加酶后脂肪分解活性即刻开始，而产品从基材膜上的释放等待着有利于释放的条件。介绍了一种从薄膜中去除降解产物的模型，其中包括对滞后现象的新颖解释。