In the present study, collagen hydrogel containing naringin was fabricated, characterized and used as the scaffold for peripheral nerve damage treatment. The collagen was dissolved in acetic acid, naringin added to the collagen solution, and cross-linked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide powder (EDC; 0.10 mM) to form the hydrogel. The microstructure, swelling behavior, biodegradation, and cyto/hemocompatibility of the fabricated hydrogels were assessed. Finally, the healing efficacy of the prepared collagen hydrogel loaded with naringin on the sciatic nerve crush injury was assessed in the animal model. The characterization results showed that the fabricated hydrogels have a porous structure containing interconnected pores with the average pore size of 90 µm. The degradation results demonstrated that about 70% of the primary weight of the naringin loaded hydrogel had been lost after 4 weeks of storage in PBS. The in vitro study showed that the proliferation of Schwann cells on the collagen/naringin hydrogel was higher than the control group (tissue culture plate) at both 48 and 72 h after cell seeding and even significantly higher than pure collagen 72 h after cell seeding (*p < 0.005, **p < 0.001). The animal study implied that the sciatic functional index reached to -22.13 ± 3.00 at the end of 60th days post-implantation which was statistically significant (p < 0.05) compared with the negative control (injury without the treatment) (-82.60 ± 1.06), and the pure collagen hydrogel (-59.80 ± 3.20) groups. The hot plate latency test, the compound muscle action potential, and wet weight-loss of the gastrocnemius muscle evaluation confirmed the positive effect of the prepared hydrogels on the healing process of the induced nerve injury. In the final, the histopathologic examinations depicted that the collagen/naringin hydrogel group reduced all the histological changes induced from the nerve injury and showed more resemblance to the normal sciatic nerve, with well-arranged fibers and intact myelin sheath. The overall results implied that the prepared collagen/naringin hydrogel can be utilized as a sophisticated alternative to healing peripheral nerve damages.

中文翻译：

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通过使用含有柚皮苷的I型胶原水凝胶再生坐骨神经。

在本研究中，含有柚皮苷的胶原蛋白水凝胶被制备，表征并用作周围神经损伤治疗的支架。将胶原蛋白溶解在乙酸中，将柚皮苷添加到胶原蛋白溶液中，并与1-乙基-3-（3-二甲基氨基丙基）-碳二亚胺粉末（EDC； 0.10mM）交联以形成水凝胶。评估了制备的水凝胶的微观结构，溶胀行为，生物降解和细胞/血液相容性。最后，在动物模型中评估了制备的带有柚皮苷的胶原蛋白水凝胶对坐骨神经压迫性损伤的治疗效果。表征结果表明，所制备的水凝胶具有包含相互连接的孔的多孔结构，其平均孔径为90 µm。降解结果表明，在PBS中储存4周后，约有70％带有柚皮苷的水凝胶失去了基本重量。体外研究表明，施旺细胞在胶原蛋白/柚皮素水凝胶上的增殖在细胞接种后48和72 h均高于对照组（组织培养板），甚至在细胞接种72 h后显着高于纯胶原（ * p <0.005，** p <0.001）。动物研究表明，植入后第60天末坐骨神经功能指数达到-22.13±3.00，与阴性对照组（未经治疗的损伤）相比，具有统计学意义（p <0.05）（-82.60±1.06） ，以及纯胶原蛋白水凝胶（-59.80±3.20）组。热板潜伏期测试，复合肌肉动作电位，腓肠肌的湿减肥和湿减肥证明了制备的水凝胶对神经损伤的愈合过程的积极作用。最后，组织病理学检查显示胶原蛋白/柚皮素水凝胶组减少了神经损伤引起的所有组织学变化，并显示出与正常坐骨神经的相似性，纤维排列良好且髓鞘完整。总体结果表明，所制备的胶原蛋白/柚皮素水凝胶可以用作治疗周围神经损伤的先进替代方法。组织病理学检查表明，胶原蛋白/柚皮素水凝胶组减少了神经损伤引起的所有组织学变化，并显示出与正常坐骨神经的相似性，纤维排列良好且髓鞘完整。总体结果表明，所制备的胶原蛋白/柚皮素水凝胶可以用作治疗周围神经损伤的先进替代方法。组织病理学检查显示，胶原蛋白/柚皮素水凝胶组减少了神经损伤引起的所有组织学变化，并显示出与正常坐骨神经的相似性，纤维排列良好且髓鞘完整。总体结果表明，所制备的胶原蛋白/柚皮素水凝胶可以用作治疗周围神经损伤的先进替代方法。