Intrinsically disordered proteins (IDPs) are integral part of the proteome, regulating vital biological processes. Such proteins gained further visibility due to their key role in neurodegenerative diseases and cancer. IDPs however, escape structural characterization by traditional biophysical tools owing to their extreme flexibility and heterogeneity. In this review, we discuss the advantages of native mass spectrometry (MS) in analysing the atypical conformational dynamics of IDPs and recent advances made in the field. Especially, MS studies unravelling the conformational facets of IDPs involved in neurodegenerative diseases are highlighted. The limitations and the future promises of native MS while studying IDPs have been discussed.

中文翻译：

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天然质谱在研究内在失调蛋白中的应用：特别关注神经退行性疾病。

内在无序蛋白（IDP）是蛋白质组的组成部分，调节重要的生物学过程。由于它们在神经退行性疾病和癌症中的关键作用，因此获得了进一步的可见性。但是，由于国内流离失所者的极端灵活性和异质性，他们无法通过传统的生物物理工具进行结构表征。在这篇综述中，我们讨论了本机质谱（MS）在分析IDP的非典型构象动力学方面的优势以及该领域的最新进展。特别是，MS研究揭示了涉及神经退行性疾病的IDP构象方面的研究。在研究IDP时，已经讨论了本机MS的局限性和未来前景。