In this work, wild-type and heterozygous β-thalassaemic mice were enriched with 57Fe via gastrointestinal absorption to characterize in greater detail the iron complexes then identifiable via Mössbauer spectroscopy. The 57Fe enrichment method was validated and Mössbauer spectra were obtained at 80 K from blood samples from wild-type and β-thalassaemic mice at 1, 3, 6, and 9 months of age. As expected, the haemoglobin levels of the thalassaemic mice were lower than from normal mice, indicating anaemia. Furthermore, significant amounts of ferritin-like iron were observed in the thalassaemic mice samples, which decreased with mouse age, reflecting the pattern of reticulocyte count reduction reported in the literature.

中文翻译：

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通过血液样本的Mössbauer光谱法对小鼠的57Fe富集进行β地中海贫血研究。

在这项工作中，野生型和杂合β-地中海贫血小鼠通过胃肠道吸收富集了57Fe，以更详细地表征铁复合物，然后通过穆斯堡尔光谱法鉴定。验证了57Fe富集方法，并在80 K下从1、3、6和9个月大的野生型和β-地中海贫血小鼠的血样中获得了Mössbauer光谱。如预期的那样，地中海贫血小鼠的血红蛋白水平低于正常小鼠，表明存在贫血。此外，在地中海贫血小鼠样品中观察到大量的铁蛋白样铁，随着小鼠年龄的增加而降低，这反映了文献报道的网织红细胞计数减少的模式。