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Transcriptome Analysis and Differential Expression in Tall Fescue Harboring Different Endophyte Strains in Response to Water Deficit.
The Plant Genome ( IF 3.9 ) Pub Date : 2019-06-01 , DOI: 10.3835/plantgenome2018.09.0071
Randy D. Dinkins 1 , Padmaja Nagabhyru 2 , Carolyn A. Young 3 , Charles P. West 4 , Christopher L. Schardl 2
Affiliation  

Two tall fescue [Lolium arundinaceum (Schreb.) Darbysh. = Schedonorus arundinaceus (Schreb.) Dumort. = Festuca arundinacea var. arundinacea Schreb.] plant genotypes with an Epichloë coenophiala (Morgan‐Jones & W. Gams) C.W. Bacon & Schardl common toxic endophyte (CTE), one with a nontoxic strain (NTE19) and one with another Epichloë species (FaTG‐4) were evaluated and compared with their respective endophyte‐free clones for responses to water‐deficit stress in the greenhouse. One of the plant genotypes (P27) showed a positive effect of its CTE strain on tiller production after stress and resumed watering. In transcriptome analysis of the pseudostems (leaf sheath whorls), differentially expressed genes (DEGs) were defined as having at least twofold expression difference and false discovery rate (FDR) < 0.05 in comparisons of water treatment (stressed or watered), endophyte presence or absence, or both. Stress affected 38% of the plant transcripts including those for the expected stress‐response pathways. The DEGs affected by endophyte in stressed plants were unique to individual plant genotypes. In unstressed plants, endophyte presence tended to reduce expression of genes putatively for defense against fungi, but in unstressed P27 endophyte presence there was enhanced expression of dehydrin and heat shock protein genes. Our results indicated subtle and variable effects of endophytes on tall fescue gene expression; where the endophyte confers protection, its effects on plant gene expression may help prime the plant for stress resistance.

中文翻译:

响应水分亏缺的高羊茅内含不同内生菌菌株的转录组分析和差异表达。

两个高大的羊茅[黑麦草(Schreb。)Darbysh。= Schedonorus arundinaceus(Schreb。)Dumort。= Festuca arundinacea var。]的基因型是带有上皮表皮(Echchloëcoenophiala)(摩根·琼斯(Morgan-Jones&W. Gams))的CW培根和夏尔特普通有毒内生菌(CTE),一种具有无毒菌株(NTE19),一种具有另一种上皮物种(FaTG-4)。评估并与它们各自的无内生菌克隆进行比较,以应对温室中的缺水胁迫。一种植物基因型(P27)在胁迫后恢复浇水,其CTE菌株对分till产量产生积极影响。在假茎(叶鞘螺纹)的转录组分析中,差异表达基因(DEG)被定义为具有至少两倍的表达差异和错误发现率(FDR)<0。比较水处理(压力或浇水),是否存在内生菌或两者兼而有之为05。胁迫影响了38%的植物转录本,包括预期的胁迫反应途径的转录本。受胁迫的植物受内生菌影响的DEG对于单个植物基因型是唯一的。在未胁迫的植物中,内生菌的存在往往会降低可能防御真菌的基因的表达,但是在未胁迫的P27内生菌的存在中,脱水素和热休克蛋白基因的表达会增强。我们的结果表明内生菌对高羊茅基因表达的微妙和可变影响。如果内生菌具有保护作用,则其对植物基因表达的作用可能有助于使植物抗逆。胁迫影响了38%的植物转录本,包括预期的胁迫反应途径的转录本。受胁迫的植物受内生菌影响的DEG对于单个植物基因型是唯一的。在未胁迫的植物中,内生菌的存在往往会降低可能防御真菌的基因的表达,但是在未胁迫的P27内生菌的存在中,脱水素和热休克蛋白基因的表达会增强。我们的结果表明内生菌对高羊茅基因表达的微妙和可变影响。如果内生菌具有保护作用,则其对植物基因表达的作用可能有助于使植物抗逆。胁迫影响了38%的植物转录本,包括预期的胁迫反应途径的转录本。受胁迫的植物受内生菌影响的DEG对于单个植物基因型是唯一的。在未胁迫的植物中,内生菌的存在往往会降低可能防御真菌的基因的表达,但是在未胁迫的P27内生菌的存在中,脱水素和热休克蛋白基因的表达会增强。我们的结果表明内生菌对高羊茅基因表达的微妙和可变影响。如果内生菌具有保护作用,则其对植物基因表达的作用可能有助于使植物抗逆。内生菌的存在往往会减少可能抵御真菌的基因的表达,但在无压力的P27内生菌的存在中,脱水素和热休克蛋白基因的表达会增强。我们的结果表明内生菌对高羊茅基因表达的微妙和可变影响。如果内生菌具有保护作用,则其对植物基因表达的作用可能有助于使植物抗逆。内生菌的存在往往会减少可能抵御真菌的基因的表达,但在无压力的P27内生菌的存在中,脱水素和热休克蛋白基因的表达会增强。我们的结果表明内生菌对高羊茅基因表达的微妙和可变影响。如果内生菌具有保护作用,则其对植物基因表达的作用可能有助于使植物抗逆。
更新日期:2019-06-01
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