Fusarium graminearum Schwabe [teleomorph: Gibberella zeae (Schweintiz) Petch] has been identified as a pathogen of soybean [Glycine max (L.) Merr.] causing seed, seedling damping‐off and root rot in North America. A major quantitative disease resistance locus (QDRL) that contributed 38.5% of the phenotypic variance toward F. graminearum in soybean was previously identified through mapping of a recombinant inbred line (RIL) population derived from a cross between ‘Wyandot’ and PI 567301B. This major QDRL mapped to chromosome 8 to a predicted 305 kb region harboring 36 genes. This locus maps near the Rhg4 locus for soybean cyst nematode (SCN) and the I locus contributing to seed coat color. Long‐read sequencing of the region was completed and variations in gene sequence and gene order compared with the ‘Williams 82’ reference were identified. Molecular markers were developed for genes within this region and mapped in the original population, slightly narrowing the region of interest. Analyses of the hybrid genome reassembly using three previously published bacterial artificial chromosome (BAC) sequences (BAC56G2, BAC104J7, and BAC77G7‐a) combined with RNA‐sequencing narrowed the region making candidate gene identification possible. The markers within this region may be used for marker‐assisted selection (MAS). There were 10 differentially expressed genes between resistant and susceptible lines, with four of these candidates also located within the genomic interval defined by the flanking markers. These genes included an actin‐related protein 2/3 complex subunit, an unknown protein, a hypothetical protein, and a chalcone synthase 3.

中文翻译：

---

大豆中针对禾谷镰刀菌的主要定量抗病基因座的杂交基因组组装。


Fusarium graminearum Schwabe [有性型：Gibberella zeae (Schweintiz) Petch] 已被鉴定为北美大豆 [ Glycine max (L.) Merr.] 的病原体，可导致种子、幼苗猝倒和根腐病。先前通过对源自“Wyandot”和 PI 567301B 杂交的重组自交系 (RIL) 群体作图，鉴定了大豆中对禾谷镰刀菌表型变异贡献 38.5% 的主要定量抗病基因座 (QDRL)。这个主要的 QDRL 映射到 8 号染色体，预计包含 36 个基因的 305 kb 区域。该基因座位于大豆胞囊线虫 (SCN) 的Rhg4基因座和影响种皮颜色的I基因座附近。该区域的长读长测序已完成，并鉴定出与“Williams 82”参考相比基因序列和基因顺序的变化。针对该区域内的基因开发了分子标记，并在原始群体中进行了绘制，稍微缩小了感兴趣的区域。使用先前发表的三个细菌人工染色体 (BAC) 序列（BAC56G2、BAC104J7 和 BAC77G7-a）结合 RNA 测序对混合基因组重组装进行分析，缩小了区域范围，使候选基因鉴定成为可能。该区域内的标记可用于标记辅助选择（MAS）。抗性品系和易感品系之间存在 10 个差异表达基因，其中 4 个候选基因也位于由侧翼标记定义的基因组区间内。这些基因包括肌动蛋白相关蛋白 2/3 复合体亚基、未知蛋白、假设蛋白和查尔酮合酶 3。