Single mammalian neurons can be isolated with adherent functional synaptic terminals using an enzyme-free, mechanical dissociation procedure. This allows investigations of the effects of presynaptic modulators of synaptic transmission with unprecedented ease and accuracy. Furthermore, single presynaptic terminals and boutons can be visualized using fluorescent markers and can also be focally stimulated with electrical pulses. In this article, the isolated-nerve-adherent-synaptic-bouton preparation and some examples of its general properties and uses are discussed.

中文翻译：

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技术：神经胸腺制剂在神经药理学中的应用。

单个哺乳动物神经元可以使用无酶，机械解离程序与附着的功能性突触末端分离。这允许以前所未有的简便性和准确性研究突触传递前突触调节剂的作用。此外，单个突触前的末端和钮扣可以使用荧光标记物进行可视化，也可以通过电脉冲进行局部刺激。在本文中，讨论了离体神经粘附突触胸腺制剂及其一些一般性质和用途的例子。