Background N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research. The large-scale characterization of N-linked glycoproteins accomplished by mass spectrometry-based glycoproteomics has provided valuable insights into the interdependence of glycoprotein structure and protein function. However, these studies focused mainly on the analysis of specific sample type, and lack the integration of glycoproteomic data from different tissues, body fluids or cell types. Methods In this study, we collected the human glycosite-containing peptides identified through their de-glycosylated forms by mass spectrometry from over 100 publications and unpublished datasets generated from our laboratory. A database resource termed N-GlycositeAtlas was created and further used for the distribution analyses of glycoproteins among different human cells, tissues and body fluids. Finally, a web interface of N-GlycositeAtlas was created to maximize the utility and value of the database. Results The N-GlycositeAtlas database contains more than 30,000 glycosite-containing peptides (representing > 14,000 N-glycosylation sites) from more than 7200 N-glycoproteins from different biological sources including human-derived tissues, body fluids and cell lines from over 100 studies. Conclusions The entire human N-glycoproteome database as well as 22 sub-databases associated with individual tissues or body fluids can be downloaded from the N-GlycositeAtlas website at http://nglycositeatlas.biomarkercenter.org.

中文翻译：

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N-GlycositeAtlas：基于质谱的人类 N-连接糖蛋白和糖基化位点定位的数据库资源。


背景 N-连接糖蛋白是临床和生物学研究中非常有趣的一类蛋白质。通过基于质谱的糖蛋白质组学完成的 N-连接糖蛋白的大规模表征为糖蛋白结构和蛋白质功能的相互依赖性提供了有价值的见解。然而，这些研究主要集中于特定样本类型的分析，缺乏来自不同组织、体液或细胞类型的糖蛋白组数据的整合。方法在这项研究中，我们收集了通过质谱法从我们实验室生成的 100 多篇出版物和未发表的数据集中通过其去糖基化形式鉴定的人类含糖位点肽。创建了名为 N-GlycositeAtlas 的数据库资源，并进一步用于不同人体细胞、组织和体液中糖蛋白的分布分析。最后，创建了N-GlycositeAtlas的Web界面，以最大化数据库的效用和价值。结果 N-GlycositeAtlas 数据库包含超过 30,000 个含糖基化位点的肽（代表 > 14,000 个 N-糖基化位点），来自不同生物来源的 7200 多个 N-糖蛋白，包括来自 100 多项研究的人源组织、体液和细胞系。结论 整个人类 N-糖蛋白质组数据库以及与个体组织或体液相关的 22 个子数据库可以从 N-GlycositeAtlas 网站 http://nglusiteatlas.biomarkercenter.org 下载。