There is growing interest in the effects of extremely low-frequency electromagnetic fields on mechanisms in biological organisms. This study's goal is to determine the role of the Nitiric Oxide (NO) pathway for thermal pain by intentionally interfering with it using a pulsed electromagnetic field generated by an extremely low-frequency alternating current (ELF-PEMF) in combination with BAY41-2272 (sGC activator), NOS inhibitor l-NAME, and NO donor l-arginine. This study included 72 adult male Wistar albino rats (mean weight of 230 ± 12 g). The rats were kept at room temperature (22 ± 2 °C) in a 12-h light/dark cycle and in a room with sound insulation. PEMF (50 Hz, 5 mT) were applied four times a day for 30 min and at 15-min intervals for 15 days. Analgesic effects were assessed with tail-flick and hot-plate tests. Before the tests, NO donor l-arginine (300 mg/kg), sGC activator BAY41-2272 (10 mg/kg), and NOS inhibitor l-name (40 mg/kg) were injected intraperitoneally into rats in six randomly-selected groups. The maximum analgesic effect of a 5 mT electromagnetic field was on day 7. PEMF significantly increased the analgesia effect when the functioning of the NO pathway was ensured with l-arginine, which is a NO donor, and BAY41-2271, which is the intracellular receptor and sGC activator. However, there was no difference between rats treated with PEMF and the NOS inhibitor l-NAME as compared to rats only treated with PEMF. In conclusion, PEMF generate analgesia by activating the NO pain pathway.

中文翻译：

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极低频脉冲电磁场​​对一氧化氮途径镇痛的影响。

人们越来越关注极低频电磁场对生物体机制的影响。这项研究的目的是通过使用极低频交流电（ELF-PEMF）与BAY41-2272结合产生的脉冲电磁场​​来故意干扰一氧化氮（NO）通路对热痛的作用， sGC激活剂），NOS抑制剂1-NAME和NO供体1-精氨酸。这项研究包括72只成年雄性Wistar白化病大鼠（平均体重230±12 g）。将大鼠在室温（22±2°C）下以12小时的明/暗周期和隔声的房间饲养。每天四次使用PEMF（50 Hz，5 mT），持续30分钟，间隔15分钟，持续15天。通过甩尾和热板试验评估镇痛效果。测试之前 在六个随机选择的组中，将腹膜内注射NO供体的l-精氨酸（300 mg / kg），sGC活化剂BAY41-2272（10 mg / kg）和NOS抑制剂l-名字（40 mg / kg）。5 mT电磁场的最大镇痛作用是在第7天。当使用NO供体的l-精氨酸和细胞内的BAY41-2271确保NO通路的功能时，PEMF可以显着提高镇痛作用。受体和sGC激活剂。然而，与仅用PEMF治疗的大鼠相比，用PEMF治疗的大鼠和NOS抑制剂1-NAME之间没有差异。总之，PEMF通过激活NO疼痛途径产生镇痛作用。5 mT电磁场的最大镇痛作用是在第7天。当使用NO供体的l-精氨酸和细胞内的BAY41-2271确保NO通路的功能时，PEMF可以显着提高镇痛作用。受体和sGC激活剂。然而，与仅用PEMF治疗的大鼠相比，用PEMF治疗的大鼠和NOS抑制剂1-NAME之间没有差异。总之，PEMF通过激活NO疼痛途径产生镇痛作用。5 mT电磁场的最大镇痛作用是在第7天。当使用NO供体的l-精氨酸和细胞内的BAY41-2271确保NO通路的功能时，PEMF可以显着提高镇痛作用。受体和sGC激活剂。然而，与仅用PEMF治疗的大鼠相比，用PEMF治疗的大鼠和NOS抑制剂1-NAME之间没有差异。总之，PEMF通过激活NO疼痛途径产生镇痛作用。与仅用PEMF治疗的大鼠相比，用PEMF治疗的大鼠和NOS抑制剂1-NAME之间没有差异。总之，PEMF通过激活NO疼痛途径产生镇痛作用。与仅用PEMF治疗的大鼠相比，用PEMF治疗的大鼠和NOS抑制剂1-NAME之间没有差异。总之，PEMF通过激活NO疼痛途径产生镇痛作用。