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The Vitamin D Receptor Gene Polymorphisms in Asthmatic Children: A Case-Control Study.
Pediatric Allergy, Immunology, and Pulmonology ( IF 0.9 ) Pub Date : 2019-06-17 , DOI: 10.1089/ped.2018.0948
Mehmet Kilic 1 , Sema Ecin 2 , Erdal Taskin 3 , Askin Sen 4 , Murat Kara 4
Affiliation  

Background: The association between vitamin D receptor (VDR) polymorphisms and the risk of asthma remains unclear. This study aimed to investigate the effect of VDR gene polymorphisms and VDR mRNA expression levels on respiratory function, nitric oxide levels in expiratory air, and serum vitamin D levels in children with asthma. Materials and Methods: The study included 80 healthy children (control group) and 100 asthmatic children (asthma group) between the age of 5 and 18 years. The VDR genotypes (ApaI, TaqI, and FokI) and VDR mRNA levels were determined in all groups. Results: There was no statistically significant difference in vitamin D levels between the asthma group and the control group (P > 0.05). A significant association was found between both genotype (CC) of the TaqI polymorphism [odds ratio (OR) = 0.2, 95% confidence interval (CI) (0.07-0.5), P = 0.003] and genotype (CA) of ApaI polymorphisms [OR = 0.2, 95% CI (0.07-0.8), P = 0.02], and asthma risk. In addition, when single-nucleotide polymorphism allelic frequencies between asthma and control groups were compared there is no significant association (P > 0.05). When compared to control group, VDR mRNA expression in asthma group decreased in genotypes CC and CA of ApaI and in genotypes TT and TC of TaqI (P < 0.05). Conclusion: The results provide supporting evidence for an association between TaqI and ApaI polymorphisms and asthma susceptibility.

中文翻译:

哮喘儿童中维生素D受体基因多态性的病例对照研究。

背景:维生素D受体(VDR)多态性与哮喘风险之间的关联仍不清楚。这项研究旨在调查VDR基因多态性和VDR mRNA表达水平对哮喘患儿呼吸功能,呼气中一氧化氮水平和血清维生素D水平的影响。资料和方法:该研究包括80名5至18岁的健康儿童(对照组)和100名哮喘儿童(哮喘组)。在所有组中确定了VDR基因型(ApaI,TaqI和FokI)和VDR mRNA水平。结果:哮喘组和对照组之间的维生素D水平无统计学差异(P> 0.05)。TaqI多态性的两种基因型(CC)之间均存在显着相关性[比​​值比(OR)= 0.2,95%的置信区间(CI)(0.07-0.5),P = 0.003]和ApaI多态性的基因型(CA)[OR = 0.2,95%CI(0.07-0.8),P = 0.02]和哮喘风险。此外,比较哮喘与对照组之间的单核苷酸多态性等位基因频率没有显着相关性(P> 0.05)。与对照组相比,哮喘组的VDR mRNA表达在ApaI的CC和CA基因型以及TaqI的TT和TC基因型中降低(P <0.05)。结论:该结果为TaqI和ApaI多态性与哮喘易感性之间的关联提供了支持证据。比较哮喘与对照组之间的单核苷酸多态性等位基因频率无显着相关性(P> 0.05)。与对照组相比,哮喘组的VDR mRNA表达在ApaI的CC和CA基因型以及TaqI的TT和TC基因型中降低(P <0.05)。结论:该结果为TaqI和ApaI多态性与哮喘易感性之间的关联提供了支持证据。比较哮喘与对照组之间的单核苷酸多态性等位基因频率无显着相关性(P> 0.05)。与对照组相比,哮喘组的VDR mRNA表达在ApaI的CC和CA基因型以及TaqI的TT和TC基因型中降低(P <0.05)。结论:该结果为TaqI和ApaI多态性与哮喘易感性之间的关联提供了支持性证据。
更新日期:2019-11-01
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