Interleukin‐24 is a pleiotropic immunoregulatory cytokine and a member of the IL‐20R subfamily of the IL‐10 family. The aim of this study was to investigate the regulation of IL‐24 in the human oral keratinocyte cell line HOK‐16B following infection with Tannerella forsythia, a major periodontal pathogen. T. forsythia induced the expression of IL‐24 mRNA and the secretion of glycosylated IL‐24 in HOK‐16B cells. Glycosylation of IL‐24 is linked to its solubility and bioavailability. T. forsythia‐stimulated reactive oxygen species (ROS) induced the expression of IL‐24, which was regulated by IL‐6. The ROS inhibitor N‐acetylcysteine and MAPK inhibitors significantly reduced the expression of IL‐6 and IL‐24 induced by T. forsythia. Recombinant human IL‐24 significantly enhanced the expression of IL‐1α, IL‐8, CXCL10, and MCP‐1 in HOK‐16B cells. Together, these results indicate that ROS, MAPKs, and IL‐6 comprise the axis of IL‐24 expression in HOK‐16B cells stimulated with T. forsythia. Thus, IL‐24 may be involved in inflammation in oral keratinocytes.

中文翻译：

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Tannerella连翘刺激的人口腔角质形成细胞中IL-24的调节。

白细胞介素24是多效性免疫调节细胞因子，是IL-10家族IL-20R亚家族的成员。这项研究的目的是研究人牙周病原菌坦氏菌连翘感染后人口腔角质形成细胞HOK-16B细胞中IL-24的调控。连翘可诱导HOK-16B细胞中IL-24 mRNA的表达和糖基化IL-24的分泌。IL-24的糖基化与其溶解度和生物利用度有关。连翘刺激的活性氧（ROS）诱导了IL-24的表达，该表达受IL-6调节。ROS抑制剂N-乙酰半胱氨酸和MAPK抑制剂显着降低连翘T诱导的IL-6和IL-24的表达。重组人IL-24可以显着增强HOK-16B细胞中IL-1α，IL-8，CXCL10和MCP-1的表达。这些结果在一起表明，ROS，MAPK和IL-6构成了由连翘刺激的HOK-16B细胞中IL-24表达的轴。因此，IL-24可能与口腔角质形成细胞的炎症有关。