To investigate the involvement of peripheral adenosine receptors in the effect of electroacupuncture (EA) on visceral pain in mice with inflammatory bowel disease (IBD). 2,4,6-Trinitrobenzene sulfonic acid (TNBS) was used to induce the visceral pain model. EA (1 mA, 2 Hz, 30 min) treatment was applied to bilateral acupoints “Dachangshu” (BL25) 1 day after TNBS injection once daily for 7 consecutive days. Von Frey filaments were used to measure the mechanical pain threshold. Western blot was used to detect the protein expression levels of adenosine 1 receptor (A1R), adenosine 2a receptor (A2aR), adenosine 2b receptor (A2bR), adenosine 3 receptor (A3R), substance P (SP), and interleukin 1 beta (IL-1β) in colon tissue. EA significantly ameliorated the disease-related indices and reduced the expression of SP and IL-1β in the colon tissues of mice with IBD. EA increased the expression of A1R, A2aR, and A3R and decreased the expression of A2bR in the colon tissue. Furthermore, the administration of adenosine receptor antagonists influenced the effect of EA. EA can inhibit the expression of the inflammatory factors SP and IL-1β by regulating peripheral A1, A2a, A2b, and A3 receptors, thus inhibiting visceral pain in IBD mice.

中文翻译：

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电针通过炎症性肠病小鼠通过腺苷受体抑制内脏疼痛。

调查外周腺苷受体参与电针（EA）对炎症性肠病（IBD）小鼠内脏痛的影响。2,4,6-三硝基苯磺酸（TNBS）用于诱导内脏疼痛模型。每天一次，连续7天，每天TNBS注射1天后，对双侧穴位“大厂鼠”（BL25）进行EA（1 mA，2 Hz，30分钟）处理。冯·弗雷（Von Frey）细丝用于测量机械疼痛阈值。Western印迹用于检测腺苷1受体（A1R），腺苷2a受体（A2aR），腺苷2b受体（A2bR），腺苷3受体（A3R），物质P（SP）和白介素1 beta（ IL-1β）。EA明显改善了IBD小鼠结肠组织中疾病相关指标，并降低了SP和IL-1β的表达。EA增加结肠组织中A1R，A2aR和A3R的表达，并降低A2bR的表达。此外，腺苷受体拮抗剂的施用影响了EA的效果。EA可以通过调节周围的A1，A2a，A2b和A3受体来抑制炎症因子SP和IL-1β的表达，从而抑制IBD小鼠的内脏痛。