Although simultaneous supplementation with iron and folic acid is justified, the potential interactions between these micronutrients are unknown. The aim of this study was to determine the effects of oral iron and folic acid, administered together or separately, on iron concentration in tissues in rats with a deficiency of both these micronutrients. In the first stage of the experiment (28 days), 150 8-week-old female Wistar rats were randomly assigned to a control group (C; n = 30) fed the standard diet and to a study group (n = 120) fed a diet deficit in iron and folate. The study group was then randomly divided to four groups: D group fed a deficit diet, FE group fed a deficit diet with iron gluconate, the FOL group fed a deficit diet with folate acid, and the FEFOL group fed a deficit diet with iron gluconate and folate acid. After 2, 10, and 21 days of supplementation, ten animals from each group were killed. Morphological parameters were measured in whole blood. Iron concentration was assayed in serum, liver, spleen, pancreas, heart, and kidneys. Folic acid supplementation more significantly decreased iron concentrations in the pancreas and spleen than in the D group after 10 and 21 days of supplementation. Moreover, the combination of iron with folic acid markedly decreased iron levels in the liver and spleen, in comparison with iron alone, after 10 and 21 days of the experiment. In conclusion, folic acid affects iron status in female rats deficient in these micronutrients in moderate and long-term supplementation.

中文翻译：

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叶酸影响缺乏这些微量营养素的雌性大鼠的铁状态。

尽管同时补充铁和叶酸是合理的，但这些微量营养素之间的潜在相互作用尚不清楚。这项研究的目的是确定口服铁和叶酸（共同或分开）对缺乏这两种微量营养素的大鼠组织中铁浓度的影响。在实验的第一阶段（28天），将150只8周大的Wistar雌性大鼠随机分配到以标准饮食喂养的对照组（C; n = 30）和以喂养标准饮食的研究组（n = 120）铁和叶酸的饮食缺乏。然后将研究组随机分为四组：D组进食不足饮食，FE组进食缺乏饮食的葡萄糖酸铁，FOL组进食不足饮食的叶酸，FEFOL组进食不足饮食的葡萄糖酸铁和叶酸。2、10，补充21天后，每组杀死了10只动物。在全血中测量形态学参数。测定血清，肝，脾，胰腺，心脏和肾脏中的铁浓度。补充叶酸10天和21天后，叶酸补充剂比D组更显着降低胰腺和脾脏中的铁浓度。而且，与单独的铁相比，在实验10天和21天后，铁与叶酸的组合显着降低了肝脏和脾脏中的铁水平。总之，在中长期补充中，叶酸会影响缺乏这些微量营养素的雌性大鼠的铁状态。肝，脾，胰腺，心脏和肾脏。补充叶酸10天和21天后，叶酸补充剂比D组更显着降低胰腺和脾脏中的铁浓度。而且，与单独的铁相比，在实验10天和21天后，铁与叶酸的组合显着降低了肝脏和脾脏中的铁水平。总之，在中长期补充中，叶酸会影响缺乏这些微量营养素的雌性大鼠的铁状态。肝，脾，胰腺，心脏和肾脏。补充叶酸10天和21天后，叶酸补充剂比D组更显着降低胰腺和脾脏中的铁浓度。而且，与单独的铁相比，在实验10天和21天后，铁与叶酸的组合显着降低了肝脏和脾脏中的铁水平。总之，在中长期补充中，叶酸会影响缺乏这些微量营养素的雌性大鼠的铁状态。实验10天和21天后，与单独使用铁相比。总之，在中长期补充中，叶酸会影响缺乏这些微量营养素的雌性大鼠的铁状态。实验10天和21天后与单独使用铁相比。总之，在中长期补充中，叶酸会影响缺乏这些微量营养素的雌性大鼠的铁状态。