ABSTRACT Considering inherence optical properties of adjoint polyfluorenes and special functions of water-soluble conjugated glycopolymers, a triazole chain glycoconjugate via one-pot method were rapidly synthesized to prepare a lactate ligand polyfluorene with a clear fluorescent label by a nickel-catalyzed Yamamoto coupling polymerization. The water solubility and biocompatibility of the glycoconjugated polymer were ameliorated when the lactose group introduced as the side chain of the conjugated polymer. As a fluorescent multivalent system of glycoconjugates containing pyranogalactose groups, the interaction between pyranogalactose group and cholera toxin B subunit was studied by fluorescence spectrophotometric titration. PF-Lac has a broad application prospect in the check of cholera toxin and the study of glycoprotein interaction.

中文翻译：

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用于检测霍乱毒素的水溶性荧光聚合物糖复合物的合成

摘要：考虑伴随聚芴的固有光学性质和水溶性共轭糖聚合物的特殊功能，通过一锅法快速合成三唑链糖缀合物，通过镍催化的山本偶联聚合制备具有清晰荧光标记的乳酸酯配体聚芴。当乳糖基团作为共轭聚合物的侧链引入时，共轭聚合物的水溶性和生物相容性得到改善。作为含有吡喃半乳糖基团的荧光多价体系，通过荧光分光光度滴定法研究了吡喃半乳糖基团与霍乱毒素B亚基之间的相互作用。PF-Lac在霍乱毒素的检测和糖蛋白相互作用的研究中具有广阔的应用前景。