Taxane-based chemotherapy-loaded drug delivery systems have great potential for cancer treatment. The docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate (PAM-PBLG-b-TPGS) nanoparticles and the docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate) (PAM-PBLG) nanoparticles were designed using a modified nanoprecipitation method. The particle size, encapsulation efficiency (EE), and in vitro release characteristics of the nanoparticles were tested. The effects of the two nanoparticles on the cellular uptake and cell viability on human cervical cancer cell line Hela and the human breast cancer cell line MCF-7 were compared. Furthermore, their antitumor efficiency was evaluated through in vivo tumour growth experiment in comparison with free DTX. PAM-PBLG-b-TPGS nanoparticles displayed high EE, smaller diameter, and a nice releasing profile. Besides, based on the high EE and ‘self-controlled’ drug release of the DTX-loaded PAM-PBLG-b-TPGS nanoparticles, they exhibited stronger cytotoxicity (lower survival rate) and higher uptake rate than DTX-loaded PAM-PBLG nanoparticles in Hela cells and MCF-7 cells. Furthermore, compared with DTX-loaded PAM-PBLG nanoparticles and free DTX, DTX-loaded PAM-PBLG-b-TPGS nanoparticles produced a potent anti-tumour effect. Thus, the DTX-loaded PAM-PBLG-b-TPGS nanoparticles provide a novel attractive nanocarrier for the DTX delivery of chemotherapy to human breast cancer cells and human cervical cancer cells.

基于紫杉烷的载有化学药物的给药系统在癌症治疗方面具有巨大潜力。装载多西他赛（DTX）的PAMAM基聚（γ-苄基-1-谷氨酸）-b - d -α-生育酚聚乙二醇1000琥珀酸酯（PAM-PBLG-b-TPGS）纳米颗粒和多西他赛（DTX）装载使用改进的纳米沉淀方法设计了基于PAMAM的聚（γ-苄基-1-谷氨酸）（PAM-PBLG）纳米颗粒。粒径，包封效率（EE）和体外测试了纳米颗粒的释放特性。比较了两种纳米颗粒对人宫颈癌细胞系Hela和人乳腺癌细胞系MCF-7的细胞摄取和细胞活力的影响。此外，通过体内评估了它们的抗肿瘤功效肿瘤生长实验与游离DTX的比较。PAM-PBLG-b-TPGS纳米颗粒显示出高EE，较小的直径和良好的释放特性。此外，基于载有DTX的PAM-PBLG-b-TPGS纳米颗粒的高EE和“自控”药物释放，与载有DTX的PAM-PBLG纳米颗粒相比，它们表现出更强的细胞毒性（较低的存活率）和更高的摄取率在Hela细胞和MCF-7细胞中。此外，与负载DTX的PAM-PBLG纳米颗粒和游离DTX相比，负载DTX的PAM-PBLG-b-TPGS纳米颗粒产生了强大的抗肿瘤作用。因此，负载DTX的PAM-PBLG-b-TPGS纳米颗粒为DTX将化学疗法递送至人乳腺癌细胞和人宫颈癌细胞提供了新颖的有吸引力的纳米载体。