Stem cells are increasingly being used in the course of burn treatment. As several different types of stem cells are available for the purposes, it is important to chose the most efficient and the most practicable stem cell type. The aim of this study was to compare the potential of heterogeneous amnion cell mixture with the presently used standard therapy, the adipose tissue-derived stem cells. The placenta was collected during a Cesarean section procedure. Adipose tissue tissue-derived cells were isolated using the Cytori's Celution® System. Cells were tested for fulfillment of the minimum criteria for stem cells. The efficiency of cell cultures was tested by an analysis of population doubling, cell proliferation, cell cycle and cell migration. Amniotic cells presented a higher ability for differentiation to chondrocytes and osteocytes than adipose-derived regenerative cells but a lower ability for differentiation toward adipocytes. Additionally, in vitro experiments have demonstrated a higher applicability of amniotic cells than adipose tissue-derived stem cells. Amniotic cells show several advantages: easy access to placenta, low costs and a lack of ethical dilemmas related to stem cell harvesting. The main disadvantage is, however, their availability, as isogenic treatment would only be possible for women around children-bearing age, unless personalized banks for amniotic cells would be established.

中文翻译：

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羊水的异质混合比脂肪组织更可能是干细胞的更好来源。

在烧伤治疗过程中越来越多地使用干细胞。由于有几种不同类型的干细胞可用于此目的，因此选择最有效和最实用的干细胞类型非常重要。这项研究的目的是比较异种羊膜细胞混合物与目前使用的标准疗法，脂肪组织来源的干细胞的潜力。剖宫产过程中收集胎盘。使用Cytori'sCelution®System分离脂肪组织组织来源的细胞。测试细胞是否满足干细胞的最低标准。通过分析种群倍增，细胞增殖，细胞周期和细胞迁移来测试细胞培养的效率。与脂肪来源的再生细胞相比，羊膜细胞向软骨细胞和骨细胞的分化能力更高，而向脂肪细胞的分化能力则更低。另外，体外实验证明羊膜细胞比脂肪组织干细胞具有更高的适用性。羊膜细胞显示出几个优点：容易获得胎盘，成本低以及缺乏与干细胞收获有关的道德困境。但是，主要的缺点是它们的可用性，因为只有在育龄妇女附近才有可能进行同基因治疗，除非建立个性化的羊膜库。体外实验证明羊膜细胞比脂肪组织干细胞具有更高的适用性。羊膜细胞显示出几个优点：容易获得胎盘，成本低以及缺乏与干细胞收获有关的道德困境。但是，主要的缺点是它们的可用性，因为只有在育龄妇女附近才有可能进行同基因治疗，除非建立个性化的羊膜库。体外实验证明羊膜细胞比脂肪组织干细胞具有更高的适用性。羊膜细胞显示出几个优点：容易获得胎盘，成本低以及缺乏与干细胞收获有关的道德困境。但是，主要的缺点是它们的可用性，因为只有在育龄妇女附近才有可能进行同基因治疗，除非建立个性化的羊膜库。