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Cationic polystyrene nanoparticle and the sea urchin immune system: biocorona formation, cell toxicity, and multixenobiotic resistance phenotype.
Nanotoxicology ( IF 3.6 ) Pub Date : 2018-06-28 , DOI: 10.1080/17435390.2018.1482378
L F Marques-Santos 1 , G Grassi 2 , E Bergami 2 , C Faleri 3 , T Balbi 4 , A Salis 5 , G Damonte 5 , L Canesi 4 , I Corsi 2
Affiliation  

In order to assess the impact of nanoplastics on marine species, polystyrene nanoparticles (PS NPs) have been largely used as model particles. Here we studied the effects of 50 nm amino-modified PS-NH2 on Mediterranean sea urchin Paracentrotus lividus immune system cells (coelomocytes) in the presence of celomic fluid (CF) and at different NP concentrations (1, 5, 10, and 25 μg mL-1) and experimental conditions (absence or presence of EDTA). PS-NH2 acquired a protein corona once incubated with CF, dominated by the toposome precursor protein (TPP). In short-term cultures, a significant concentration- and time-dependent decrease in lysosomal membrane stability and apoptotic-like nuclear alterations were observed in phagocytes upon exposure to PS-NH2 (10 and 25 µg mL-1) in CF but they resulted abolished in the presence of EDTA confirming the role of TPP in triggering PS-NH2-coelomocytes interaction and toxicity. PS-NH2 did not alter MXR phenotype but the observed dose-dependent decrease in calcein accumulation suggests the ability of PS-NH2 to affect pump's efflux activity. Overall results encourage additional studies on positively charged nanoplastics, since the observed effects on sea urchin coelomocytes as well as the TPP corona formation might represent a first step for addressing their impact on sensitive marine species.

中文翻译:

阳离子聚苯乙烯纳米颗粒和海胆免疫系统:生物电晕形成,细胞毒性和多异生物抗性表型。

为了评估纳米塑料对海洋物种的影响,聚苯乙烯纳米颗粒(PS NP)已被广泛用作模型颗粒。在这里,我们研究了在存在体温液体(CF)和不同NP浓度(1、5、10和25μg)的情况下,50 nm氨基修饰的PS-NH2对地中海海胆Paracentrotus lividus免疫系统细胞(结肠细胞)的影响mL-1)和实验条件(不存在或存在EDTA)。PS-NH2一旦与CF一起孵育,就获得了蛋白冠状蛋白,该蛋白冠状体是前体蛋白(TPP)支配的。在短期文化中 在CF中暴露于PS-NH2(10和25 µg mL-1)后,吞噬细胞中溶酶体膜的稳定性显着下降,并随时间变化,并呈凋亡样核变化,但在EDTA的存在下它们消失了。 TPP在触发PS-NH2-内皮细胞相互作用和毒性中的作用。PS-NH2不会改变MXR表型,但观察到的钙黄绿素累积的剂量依赖性降低表明PS-NH2影响泵的外排活性的能力。总体结果鼓励对带正电荷的纳米塑料进行更多的研究,因为观察到的对海胆腔内皮细胞以及TPP电晕形成的影响可能是解决其对敏感海洋物种影响的第一步。PS-NH2不会改变MXR表型,但观察到的钙黄绿素累积的剂量依赖性降低表明PS-NH2影响泵的外排活性的能力。总体结果鼓励对带正电荷的纳米塑料进行更多的研究,因为观察到的对海胆腔内皮细胞以及TPP电晕形成的影响可能是解决其对敏感海洋物种影响的第一步。PS-NH2不会改变MXR表型,但观察到的钙黄绿素累积的剂量依赖性降低表明PS-NH2影响泵的外排活性的能力。总体结果鼓励对带正电荷的纳米塑料进行更多的研究,因为观察到的对海胆腔内皮细胞以及TPP电晕形成的影响可能代表着解决其对敏感海洋物种影响的第一步。
更新日期:2018-06-27
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