Gene expression involves a cascade of events, from transcription factor transactivation and RNA polymerase binding to RNA processing and maturation. mRNA profiling has yielded a vast amount of information about the total RNA content in different tissues physiological states. However, it has been unclear how the variable amounts of mRNA might correlate with transcriptional events. Recent advances in single-cell imaging offer a platform for 'cellular genomics', the high throughput analysis of transcriptional activity in single cells.

中文翻译：

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细胞基因组学：转录哪些基因，何时何地？

基因表达涉及一系列事件，从转录因子反式激活和 RNA 聚合酶结合到 RNA 加工和成熟。mRNA 分析已经产生了大量关于不同组织生理状态下总 RNA 含量的信息。然而，目前尚不清楚可变数量的 mRNA 如何与转录事件相关。单细胞成像的最新进展为“细胞基因组学”提供了一个平台，即单细胞转录活性的高通量分析。