Three major eukaryotic DNA polymerases, Polymerases α, δ, and ε (Pols α, δ, and ε), perform the fundamental process of DNA synthesis at the replication fork both accurately and efficiently. In trying to understand the necessity and flexibility of the polymerase usage, we recently reported that budding yeast cells lacking Pol ε exonuclease and polymerase domains (pol2-16) survive, but have severe growth defects, checkpoint activation, increased level of dNTP pools as well as significant increase in the mutation rates. Herein, we suggest new opportunities to distinguish the roles of Pol ε from those of two other eukaryotic B-family DNA polymerases, Pols δ and ζ.

中文翻译：

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在发芽酵母中进行核DNA复制酶学新研究的机会。

三种主要的真核DNA聚合酶，即聚合酶α，δ和ε（聚合酶α，δ和ε）在复制叉处准确而有效地执行了DNA合成的基本过程。在试图了解聚合酶用法的必要性和灵活性时，我们最近报道了缺乏Polε核酸外切酶和聚合酶结构域（pol2-16）的出芽酵母细胞可以存活，但具有严重的生长缺陷，检查点激活，dNTP库水平增加显着提高了突变率。在这里，我们建议新的机会来区分Polε和其他两个真核B族DNA聚合酶Polsδ和ζ的作用。