Reclamation of anthropogenically impacted environments is a critical issue worldwide. In the oil sands extraction industry of Alberta, reclamation of mining‐impacted areas, especially areas affected by tailings waste, is an important aspect of the mining life cycle. A reclamation technique currently under study is water‐capping, where tailings are capped by water to create an end‐pit lake (EPL). Base Mine Lake (BML) is the first full‐scale end‐pit lake in the Alberta oil sands region. In this study, we sequenced eukaryotic 18S rRNA genes recovered from 92 samples of Base Mine Lake water in a comprehensive sampling programme covering the ice‐free period of 2015. The 565 operational taxonomic units (OTUs) generated revealed a dynamic and diverse community including abundant Microsporidia, Ciliata and Cercozoa, though 41% of OTUs were not classifiable below the phylum level by comparison to 18S rRNA databases. Phylogenetic analysis of five heterotrophic phyla (Cercozoa, Fungi, Ciliata, Amoebozoa and Excavata) revealed substantial novel diversity, with many clusters of OTUs that were more similar to each other than to any reference sequence. All of these groups are entirely or mostly heterotrophic, as a relatively small number of definitively photosynthetic clades were amplified from the BML samples.

中文翻译：

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阿尔伯塔北部油砂尾矿开垦基地基地矿湖群落组成和新真核生物谱系的系统发育估计

开垦受人为影响的环境是世界范围内的一个关键问题。在艾伯塔省的油砂开采行业，受采矿影响地区，特别是受尾矿废物影响的地区的复垦是采矿生命周期的一个重要方面。目前正在研究的一种复垦技术是封水技术，即尾矿被水封住以形成端坑湖 (EPL)。Base Mine Lake (BML) 是阿尔伯塔油砂地区第一个全尺寸端坑湖。在这项研究中，我们在涵盖 2015 年无冰期的综合采样计划中对从 Base Mine 湖水的 92 个样本中回收的真核 18S rRNA 基因进行了测序。 生成的 565 个操作分类单元 (OTU) 揭示了一个动态多样的群落，包括丰富的微孢子虫、纤毛虫和尾毛虫，尽管与 18S rRNA 数据库相比，41% 的 OTU 无法在门级别以下进行分类。对五个异养门（Cercozoa、Fungi、Ciliata、Amoebozoa 和 Excavata）的系统发育分析揭示了大量新的多样性，其中许多 OTU 簇彼此之间的相似度比任何参考序列都更相似。所有这些群体都完全或大部分是异养的，因为从 BML 样本中扩增出相对少量的明确光合进化枝。